MRCKβ, active
N-terminal 6His-tagged recombinant, human MRCKb amino acids 1-473.
No items found.
Biological information
Background
MRCKβ, also called CDC42 Binding Protein Kinase Beta, is a member of the serine/threonine protein kinase family. The protein contains a Cdc42/Rac-binding p21 binding domain resembling that of PAK kinase. The kinase domain of this protein is most closely related to that of myotonic dystrophy kinase-related ROK. Studies of the similar gene in rat suggested that this kinase may act as a downstream effector of Cdc42 in cytoskeletal reorganization. [provided by RefSeq, Jul 2008]
Target class
Kinase
Family
AGC
Accession number
NM_006035.3
Target Name
MRCKβ, active
Target Alias
CDC42BPB, KIAA1124, MRCKB
Origin
Human
Theori. MW
55.5 kDa
Affinity tag
6His
Product specifications
Expression system
Expressed by baculovirus in Sf21 insect cells
Purity
Purity 71.4% by SDS-PAGE and Coomassie blue staining.
Purification method
Ni2+/NTA-agarose
Sample Buffer
Protein in 50mM Tris/HCl pH7.5,
300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose,
1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol.
Frozen solution.
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
6 months at -70°C
Usage disclaimer
For Research Only
Chemical data
Background
MRCKβ, also called CDC42 Binding Protein Kinase Beta, is a member of the serine/threonine protein kinase family. The protein contains a Cdc42/Rac-binding p21 binding domain resembling that of PAK kinase. The kinase domain of this protein is most closely related to that of myotonic dystrophy kinase-related ROK. Studies of the similar gene in rat suggested that this kinase may act as a downstream effector of Cdc42 in cytoskeletal reorganization. [provided by RefSeq, Jul 2008]
Compound name
Kinase
Catalog number
14-643
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Datasheets
14-643
Datasheet