MLCK, active
N-terminal 6His-tagged recombinant, human MLCK amino acids 1425-1771.
No items found.
Biological information
Background
MYLK (myosin light polypeptide kinase; myosin light chain kinase, MLCK), which belongs to the family of Ca2+/calmodulin (CaM)-dependent protein protein kinases, induces a high degree of myosin motor activity by specifically phosphorylating the myosin II regulatory light chain (MRLC). Myosin II phosphorylation is involved in smooth muscle contraction and cell motility in non-muscle cells.
GenBank NM_053025
Target class
Kinase
Family
CAMK
Accession number
NM_053025
Target Name
MLCK, active
Target Alias
MYLK, DKFZp686I10125, MLCK108, FLJ12216, MSTP083, KRP, MLCK210, MLCK, Telokin
Origin
Human
Theori. MW
43.7 kDa
Affinity tag
6His
Product specifications
Expression system
Expressed by baculovirus in Sf21 insect cells
Purity
Refer to CoA for Purity
Purification method
Ni2+/NTA-agarose
Sample Buffer
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
6 months at -70°C
Usage disclaimer
For Research Only
Chemical data
Background
MYLK (myosin light polypeptide kinase; myosin light chain kinase, MLCK), which belongs to the family of Ca2+/calmodulin (CaM)-dependent protein protein kinases, induces a high degree of myosin motor activity by specifically phosphorylating the myosin II regulatory light chain (MRLC). Myosin II phosphorylation is involved in smooth muscle contraction and cell motility in non-muscle cells.
GenBank NM_053025
Compound name
Kinase
Catalog number
14-638
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Datasheets
14-638
Datasheet