PKC iota, active
N-terminal 6His-tagged recombinant, human full length PKC iota PKC Lipid activator (Catalog # 20-133) is required for use.
No items found.
Biological information
Background
Protein kinase C, iota (PKCiota, PRKCI) is one of the major protein kinase C isoforms of a family of serine/threonine protein kinases. Breakdown of phosphatidylinositol leads to formation of diacylglycerol and inositol triphophate, the former acting synergistically with Ca2+ to activate protein kinase C (PKC), resulting in translocation of the enzyme from cytosol to the membrane. Phosphorylation of proteins by PKC has been implicated as a pivotal regulatory element in signal transduction, cellular regulation, and with a high affinity to phorbol ester tumor promoters.
GenBank NM_002740
Target class
Kinase
Family
AGC
Accession number
NM_002740.5
Target Name
PKC iota, active
Target Alias
PRKCI, DXS1179E, MGC26534, PRKC-lambda/iota, PKCI, aPKC-lambda/iota, nPKC-iota
Origin
Human
Theori. MW
68.6 kDa
Affinity tag
6His
Product specifications
Expression system
Expressed by baculovirus in Sf21 insect cells
Purity
Refer to CoA for Purity
Purification method
Ni2+/NTA-agarose
Sample Buffer
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
6 months at -70°C
Usage disclaimer
For Research Only
Chemical data
Background
Protein kinase C, iota (PKCiota, PRKCI) is one of the major protein kinase C isoforms of a family of serine/threonine protein kinases. Breakdown of phosphatidylinositol leads to formation of diacylglycerol and inositol triphophate, the former acting synergistically with Ca2+ to activate protein kinase C (PKC), resulting in translocation of the enzyme from cytosol to the membrane. Phosphorylation of proteins by PKC has been implicated as a pivotal regulatory element in signal transduction, cellular regulation, and with a high affinity to phorbol ester tumor promoters.
GenBank NM_002740
Compound name
Kinase
Catalog number
14-505
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Datasheets
14-505
Datasheet