PKC η, active
N-terminal 6His-tagged recombinant, human PKC eta amino acids 2-end. PKC Lipid activator (Catalog # 20-133) is required for use.
No items found.
Biological information
Background
Protein kinase Ch (PKCh) is one of the major protein kinase C isoforms of a family of serine/threonine protein kinases. Breakdown of phosphatidylinositol leads to formation of diacylglycerol and inositol triphophate, the former acting synergistically with Ca2+ to activate protein kinase C (PKC), resulting in translocation of the enzyme from cytosol to the membrane. Phosphorylation of proteins by PKC has been implicated as a pivotal regulatory element in signal transduction, cellular regulation, and with a high affinity to phorbol ester tumor promoters.
GenBank NM_006255. Translation conflicts with NM_006255, V108L and G109R reported in dbEST BG719560 and BM549890, and L392Q reported in dbEST BI913495, BQ051772 and AU136862.
Target class
Kinase
Family
AGC
Accession number
NM_006255.3
Target Name
PKC η, active
Target Alias
PRKCH, PRKCL, PKCL, MGC26269, nPKC-eta, MGC5363, PKC-L
Origin
Human
Theori. MW
78.6 kDa
Affinity tag
6His
Product specifications
Expression system
Expressed by baculovirus in Sf21 insect cells
Purity
Refer to CoA for Purity
Purification method
Ni2+/NTA-agarose
Sample Buffer
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
6 months at -70°C
Usage disclaimer
For Research Only
Chemical data
Background
Protein kinase Ch (PKCh) is one of the major protein kinase C isoforms of a family of serine/threonine protein kinases. Breakdown of phosphatidylinositol leads to formation of diacylglycerol and inositol triphophate, the former acting synergistically with Ca2+ to activate protein kinase C (PKC), resulting in translocation of the enzyme from cytosol to the membrane. Phosphorylation of proteins by PKC has been implicated as a pivotal regulatory element in signal transduction, cellular regulation, and with a high affinity to phorbol ester tumor promoters.
GenBank NM_006255. Translation conflicts with NM_006255, V108L and G109R reported in dbEST BG719560 and BM549890, and L392Q reported in dbEST BI913495, BQ051772 and AU136862.
Compound name
Kinase
Catalog number
14-497
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Datasheets
14-497
Datasheet