PKC θ, active
C-terminal 6His-tagged recombinant, human full length PKC theta. PKC Lipid activator (Catalog # 20-133) is required for use.
No items found.
Biological information
Background
Protein kinase C, theta (PKCtheta, PRKCQ, PRKCT) is one of the major protein kinase C isoforms of a family of serine/threonine protein kinases. Breakdown of phosphatidylinositol leads to formation of diacylglycerol and inositol triphophate, the former acting synergistically with Ca2+ to activate protein kinase C (PKC), resulting in translocation of the enzyme from cytosol to the membrane. Phosphorylation of proteins by PKC has been implicated as a pivotal regulatory element in signal transduction, cellular regulation, and with a high affinity to phorbol ester tumor promoters.
GenBank L07032
Target class
Kinase
Family
AGC
Accession number
NM_006257.2
Target Name
PKC θ, active
Target Alias
PRKCQ, OTTHUMP00000043365, MGC126514, OTTHUMP00000043364, MGC141919, PRKCT, nPKC-theta
Origin
Human
Theori. MW
83 kDa
Affinity tag
6His
Product specifications
Expression system
Expressed by baculovirus in Sf21 insect cells
Purity
Refer to CoA for Purity
Purification method
Immobilized nickel affinity chromatography
Sample Buffer
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
6 months at -70°C
Usage disclaimer
For Research Only
Chemical data
Background
Protein kinase C, theta (PKCtheta, PRKCQ, PRKCT) is one of the major protein kinase C isoforms of a family of serine/threonine protein kinases. Breakdown of phosphatidylinositol leads to formation of diacylglycerol and inositol triphophate, the former acting synergistically with Ca2+ to activate protein kinase C (PKC), resulting in translocation of the enzyme from cytosol to the membrane. Phosphorylation of proteins by PKC has been implicated as a pivotal regulatory element in signal transduction, cellular regulation, and with a high affinity to phorbol ester tumor promoters.
GenBank L07032
Compound name
Kinase
Catalog number
14-444
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Datasheets
14-444
Datasheet