PKA, catalytic subunit, recombinant
Untagged recombinant, human full length PKA, catalytic subunit type α.
No items found.
Biological information
Background
Cyclic-AMP exerts its effects in animal cells mainly by activating the enzyme cyclic AMP-dependent protein kinase A (PKA). This enzyme catalyzes transfer of the terminal phosphate group from ATP to specific serines and threonines of selected proteins. Covalent phosphorylation of the appropriate amino acids of specific cellular proteins in turn results in altered cellullar function as a consequence of activation or inactivation of enzymes or altered membrane permeability to ions or other possible effects. PKA is found in all animal cells, and it is known that hormones or transmitters may be mediated by cAMP-stimulated phosphorylation of specific sites on neuronal or non-neuronal cells to exert their physiological function.
GenBank X07767
Target class
Kinase
Family
AGC
Accession number
NM_002730.3; NM_207518.1
Target Name
PKA, catalytic subunit, recombinant
Target Alias
PRKACA, MGC102831, PKACA, MGC48865
Origin
Human
Theori. MW
40.7 kDa
Affinity tag
Product specifications
Expression system
Expressed in E. coli BL21 (DE3) pLysS cells
Purity
Refer to CoA for Purity
Purification method
Phosphocellulose P11 chromatography
Sample Buffer
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
1 year at -70°C
Usage disclaimer
For Research Only
Chemical data
Background
Cyclic-AMP exerts its effects in animal cells mainly by activating the enzyme cyclic AMP-dependent protein kinase A (PKA). This enzyme catalyzes transfer of the terminal phosphate group from ATP to specific serines and threonines of selected proteins. Covalent phosphorylation of the appropriate amino acids of specific cellular proteins in turn results in altered cellullar function as a consequence of activation or inactivation of enzymes or altered membrane permeability to ions or other possible effects. PKA is found in all animal cells, and it is known that hormones or transmitters may be mediated by cAMP-stimulated phosphorylation of specific sites on neuronal or non-neuronal cells to exert their physiological function.
GenBank X07767
Compound name
Kinase
Catalog number
14-440
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Datasheets
14-440
Datasheet