MSK1, active (flag tag)
N-terminal 6His-tagged and FLAG-tagged recombinant, human MSK1 amino acids 2-end.
No items found.
Biological information
Background
MSK1 (mitogen- and stress-activated protein kinases-1, ribosomal protein S6 kinase, 90kDa, polypeptide 5) and MSK2 (mitogen- and stress-activated protein kinases-2, ribosomal protein S6 kinase, 90kDa, polypeptide 4) are activated in vitro by MAPK2/ERK2 or SAPK2/p38. Endogenous MSK is activated by UV radiation, oxidative stress, and other cell-damaging stimuli. MSK may mediate the growth factor and stress-induced activation of CREB.
GenBank AF074393
Target class
Kinase
Family
AGC
Accession number
AF074393
Target Name
MSK1, active (flag tag)
Target Alias
Origin
Human
Theori. MW
94 kDa
Affinity tag
6His, FLAG
Product specifications
Expression system
Expressed by baculovirus in Sf21 insect cells
Purity
Purity 83.7% by SDS-PAGE and Coomassie blue staining.
Purification method
Ni2+/NTA agarose, activated using human MAPK2, and then re-purified using Ni2+/NTA agarose
Sample Buffer
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
1 year at -70°C
Usage disclaimer
For Research Only
Chemical data
Background
MSK1 (mitogen- and stress-activated protein kinases-1, ribosomal protein S6 kinase, 90kDa, polypeptide 5) and MSK2 (mitogen- and stress-activated protein kinases-2, ribosomal protein S6 kinase, 90kDa, polypeptide 4) are activated in vitro by MAPK2/ERK2 or SAPK2/p38. Endogenous MSK is activated by UV radiation, oxidative stress, and other cell-damaging stimuli. MSK may mediate the growth factor and stress-induced activation of CREB.
GenBank AF074393
Compound name
Kinase
Catalog number
14-438
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Datasheets
14-438
Datasheet