MEK1, unactive
N-terminal GST-tagged and C-terminal 6His-tagged recombinant, human full length MEK1.
No items found.
Biological information
Background
ERKs (extracellular signal-regulated kinase) are activated by the MEK (mitogen-activated protein kinase, or ERK kinase). MEKs are activated by members of the Raf family. The signaling pathway comprising Raf and ERK (extracellular signal-regulated kinase) lies downstream of the small guanine nucleotide binding protein Ras and mediates several apparently conflicting cellular responses, such as proliferation, apoptosis, growth arrest, differentiation, and senescence, depending on the duration and strength of the external stimulus and on cell type. An inhibitor assay may be of benefit for the treatment of cancer, diabetes, neurodegenerative disorders or asthma.
EMBL Z30163, possessing amino acid substitution A327G. Please note, introduction of the A327G mutation to the rabbit sequence (Z30163) results in expression of recombinant MEK1 protein that is 100% identical to wild-type human MEK1 described in SwissProt Q02750, note this SwissProt entry fails to record M1 and starts at P2.
Target class
Kinase
Family
STE
Accession number
NM_002755.2
Target Name
MEK1, unactive
Target Alias
MAP2K1, MAPKK1, PRKMK1, MEK1, MKK1
Origin
Human
Theori. MW
71 kDa
Affinity tag
GST, 6His
Product specifications
Expression system
Expressed in E. coli
Purity
Refer to CoA for Purity
Purification method
Glutathione agarose affinity chromatography; Ni2+/NTA agarose
Sample Buffer
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
6 months at -70°C
Usage disclaimer
For Research Only
Chemical data
Background
ERKs (extracellular signal-regulated kinase) are activated by the MEK (mitogen-activated protein kinase, or ERK kinase). MEKs are activated by members of the Raf family. The signaling pathway comprising Raf and ERK (extracellular signal-regulated kinase) lies downstream of the small guanine nucleotide binding protein Ras and mediates several apparently conflicting cellular responses, such as proliferation, apoptosis, growth arrest, differentiation, and senescence, depending on the duration and strength of the external stimulus and on cell type. An inhibitor assay may be of benefit for the treatment of cancer, diabetes, neurodegenerative disorders or asthma.
EMBL Z30163, possessing amino acid substitution A327G. Please note, introduction of the A327G mutation to the rabbit sequence (Z30163) results in expression of recombinant MEK1 protein that is 100% identical to wild-type human MEK1 described in SwissProt Q02750, note this SwissProt entry fails to record M1 and starts at P2.
Compound name
Kinase
Catalog number
14-420
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Datasheets
14-420
Datasheet