PRAK, unactive
N-terminal 6His-tagged recombinant, human full length PRAK.
No items found.
Biological information
Background
Protein Serine/Threonine Kinase MAPKAPK5 (mitogen-activated protein kinase activated protein kinase 5), also known as p38-regulated/activated protein kinase (PRAK), functions downstream of p38a and p38b in mediating signaling of the p38 pathway. This kinase can be activated in response to cellular stress and proinflammatory cytokines through its phosphorylation by MAP kinases including MAPK1/ERK, MAPK14/p38-alpha, and MAPK11/p38-beta. MAPKAPK5 is localized predominantly in the cytoplasm and is constantly shuttled between the cytoplasm and the nucleus. MAPKAPK5 may play a role in balancing other MAPK pathways because overactivation of MAPKAPK5 can inhibit Ras mediated cell proliferation and gene activation. MAPKAPK5 inhibtion may facilitate cell differentiation, inflammation and cell proliferation.
EMBL AF032437. Protein is R291E with respect to this accession. This conflict is reported inGenBank AI300977, AL556217 and BI495728.
Target class
Kinase
Family
CAMK
Accession number
NM_003668.2; NM_139078.1
Target Name
PRAK, unactive
Target Alias
MAPKAPK5, PRAK
Origin
Human
Theori. MW
54 kDa
Affinity tag
6His
Product specifications
Expression system
Expressed by baculovirus in Sf21 insect cells
Purity
Refer to CoA for Purity
Purification method
Ni2+/NTA-agarose
Sample Buffer
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
6 months at -20°C
Usage disclaimer
For Research Only
Chemical data
Background
Protein Serine/Threonine Kinase MAPKAPK5 (mitogen-activated protein kinase activated protein kinase 5), also known as p38-regulated/activated protein kinase (PRAK), functions downstream of p38a and p38b in mediating signaling of the p38 pathway. This kinase can be activated in response to cellular stress and proinflammatory cytokines through its phosphorylation by MAP kinases including MAPK1/ERK, MAPK14/p38-alpha, and MAPK11/p38-beta. MAPKAPK5 is localized predominantly in the cytoplasm and is constantly shuttled between the cytoplasm and the nucleus. MAPKAPK5 may play a role in balancing other MAPK pathways because overactivation of MAPKAPK5 can inhibit Ras mediated cell proliferation and gene activation. MAPKAPK5 inhibtion may facilitate cell differentiation, inflammation and cell proliferation.
EMBL AF032437. Protein is R291E with respect to this accession. This conflict is reported inGenBank AI300977, AL556217 and BI495728.
Compound name
Kinase
Catalog number
14-335
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Datasheets
14-335
Datasheet