Lyn, active, mouse
N-terminal 6His-tagged recombinant, murine full length Lyn.
No items found.
Biological information
Background
The Lyn protein is a member of the Src family of non-receptor-associated protein-tyrosine kinases. LYN A differs from LYN B in having an additional 21 amino acids at the amino terminus. Lyn undergoes a concentration-dependent autophosphorylation, thus activating the catalytic site and decreasing the accessibility of the SH2 domain. Lyn phosphorylates and activates Tec, non-receptor tyrosine kinase that is coexpressed with Lyn and acts downstream of Lyn in intracellular signaling pathways. Lyn tyrosine kinase has been shown to be necessary for IgE-receptor (FceRI)-mediated mast-cell activation.
EMBL M64608, N425D as reported in SWISS PROT P25911
Target class
Kinase
Family
TK
Accession number
NM_002350.1
Target Name
Lyn, active, mouse
Target Alias
LYN, JTK8, FLJ26625
Origin
Mouse
Theori. MW
60 kDa
Affinity tag
6His
Product specifications
Expression system
Expressed by baculovirus in Sf21 insect cells
Purity
Refer to CoA for Purity
Purification method
Ni2+/NTA-agarose
Sample Buffer
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
1 year at -70°C
Usage disclaimer
For Research Only
Chemical data
Background
The Lyn protein is a member of the Src family of non-receptor-associated protein-tyrosine kinases. LYN A differs from LYN B in having an additional 21 amino acids at the amino terminus. Lyn undergoes a concentration-dependent autophosphorylation, thus activating the catalytic site and decreasing the accessibility of the SH2 domain. Lyn phosphorylates and activates Tec, non-receptor tyrosine kinase that is coexpressed with Lyn and acts downstream of Lyn in intracellular signaling pathways. Lyn tyrosine kinase has been shown to be necessary for IgE-receptor (FceRI)-mediated mast-cell activation.
EMBL M64608, N425D as reported in SWISS PROT P25911
Compound name
Kinase
Catalog number
14-315
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Datasheets
14-315
Datasheet