MAP Kinase 2/Erk2, active
N-terminal GST-tagged recombinant, MAP Kinase 2 activated using MEK1 (Catalog #14-429).
No items found.
Biological information
Background
MAPK1 (mitogen-activated protein kinase 1, ERK2 or extracellular signal –regulated protein kinase) is one of the isoforms of MAP kinase. This serine/threonine kinase is activated by dual phosphorylation on tyrosine and threonine residues (Tyr-185 and Thr-183). Phosphorylation of both residues by the upstream activator MEK (mitogen-activated protein kinase kinase, MAP2K) is essential not only for ERK2 activation, but also for translocation of the ERK2 to the nucleus. Following this activation, ERK2 phosphorylates a large number of regulatory proteins and this directly controls key cellular processes such as transcription, translation and cytoskeletal rearrangement. An inhibitor assay may be of benefit for the treatment of cancer, diabetes, neurodegenerative disorders or asthma.
GenBank NM_002745 (amino acid sequence only)
Target class
Kinase
Family
CMGC
Accession number
NM_002745.4; NM_138957.2
Target Name
MAP Kinase 2/Erk2, active
Target Alias
MAPK1, p40, PRKM2, MAPK2, PRKM1, p42-MAPK, p38, p41, ERK, ERK-2, ERT1, P42MAPK, p41mapk, ERK2
Origin
Human
Theori. MW
67.8 kDa
Affinity tag
GST
Product specifications
Expression system
Expressed in E. coli
Purity
Refer to CoA for Purity
Purification method
Glutathione agarose affinity chromatography; Ni2+/NTA agarose
Sample Buffer
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
1 year at -20°C
Usage disclaimer
For Research Only
Chemical data
Background
MAPK1 (mitogen-activated protein kinase 1, ERK2 or extracellular signal –regulated protein kinase) is one of the isoforms of MAP kinase. This serine/threonine kinase is activated by dual phosphorylation on tyrosine and threonine residues (Tyr-185 and Thr-183). Phosphorylation of both residues by the upstream activator MEK (mitogen-activated protein kinase kinase, MAP2K) is essential not only for ERK2 activation, but also for translocation of the ERK2 to the nucleus. Following this activation, ERK2 phosphorylates a large number of regulatory proteins and this directly controls key cellular processes such as transcription, translation and cytoskeletal rearrangement. An inhibitor assay may be of benefit for the treatment of cancer, diabetes, neurodegenerative disorders or asthma.
GenBank NM_002745 (amino acid sequence only)
Compound name
Kinase
Catalog number
14-173
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Datasheets
14-173
Datasheet