PDK1, active Human Recombinant Kinase Protein
N-terminal 6His-tagged recombinant, human PDK1 amino acids 52-end.
No items found.
Biological information
Background
Phosphoinositide-Dependent Kinase-1 (PDK1) plays a central role in mediating cell signaling between PI3K and various intracellular serine/threonine kinases by phosphorylating many other kinases including PKC, p70S6 kinase, ribosomal S6 kinase, p21-activated kinase, PKC-related kinase, PKB and serum glucocorticoid kinase. PDK1 interacts with a wide range of substrates, and has been found to regulate apoptosis, cell proliferation and survival through the PI3K signaling pathway. A PDK1 inhibitor may be of therapeutic use in the treatment of human cancers and diabetes.
GenBank AF017995
Target class
Kinase
Family
AGC
Accession number
NM_002613.3; NM_031268.4
Target Name
PDK1, active Human Recombinant Kinase Protein
Target Alias
PDPK1, MGC20087, PDK1, PkB-like, PRO0461, MGC35290, hPDK1
Origin
Human
Theori. MW
59 kDa
Affinity tag
6His
Product specifications
Expression system
Expressed by baculovirus in Sf21 insect cells
Purity
Refer to CoA for Purity
Purification method
Ni2+/NTA-agarose
Sample Buffer
Protein in 50mM Tris/HCl, pH 7.5, 0.15M NaCl, 0.1mM EGTA, 0.03% Brij-35, 0.1% 2-mercaptoethanol, 1mM benzamidine, 200μM PMSF and 5% glycerol
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
6 months at -70°C
Usage disclaimer
For Research Only
Chemical data
Background
Phosphoinositide-Dependent Kinase-1 (PDK1) plays a central role in mediating cell signaling between PI3K and various intracellular serine/threonine kinases by phosphorylating many other kinases including PKC, p70S6 kinase, ribosomal S6 kinase, p21-activated kinase, PKC-related kinase, PKB and serum glucocorticoid kinase. PDK1 interacts with a wide range of substrates, and has been found to regulate apoptosis, cell proliferation and survival through the PI3K signaling pathway. A PDK1 inhibitor may be of therapeutic use in the treatment of human cancers and diabetes.
GenBank AF017995
Compound name
Kinase
Catalog number
14-452
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Kinase Activity Assay Biological information
Background
Phosphoinositide-Dependent Kinase-1 (PDK1) plays a central role in mediating cell signaling between PI3K and various intracellular serine/threonine kinases by phosphorylating many other kinases including PKC, p70S6 kinase, ribosomal S6 kinase, p21-activated kinase, PKC-related kinase, PKB and serum glucocorticoid kinase. PDK1 interacts with a wide range of substrates, and has been found to regulate apoptosis, cell proliferation and survival through the PI3K signaling pathway. A PDK1 inhibitor may be of therapeutic use in the treatment of human cancers and diabetes.
GenBank AF017995
Target class
Kinase
Family
AGC
Subfamily
Protein Name
PDK1
Protein Alias
PDPK1, MGC20087, PDK1, PkB-like, PRO0461, MGC35290, hPDK1
Accession Number
NM_002613.3; NM_031268.4
UniProt Number
Gene Name
Gene ID
Gene Aliases
Target Species
Human
Kinase Activity Assay Usage
Product Type
Application
Storage Conditions
6 months at -70°C
Usage disclaimer
Kinase Activity Assay Information
Assay Type
Assay Measures
Biological Information
Background
Phosphoinositide-Dependent Kinase-1 (PDK1) plays a central role in mediating cell signaling between PI3K and various intracellular serine/threonine kinases by phosphorylating many other kinases including PKC, p70S6 kinase, ribosomal S6 kinase, p21-activated kinase, PKC-related kinase, PKB and serum glucocorticoid kinase. PDK1 interacts with a wide range of substrates, and has been found to regulate apoptosis, cell proliferation and survival through the PI3K signaling pathway. A PDK1 inhibitor may be of therapeutic use in the treatment of human cancers and diabetes.
GenBank AF017995
Target class
Kinase
Family
AGC
Accession Number
NM_002613.3; NM_031268.4
Target Name
PDK1
Target Alias
PDPK1, MGC20087, PDK1, PkB-like, PRO0461, MGC35290, hPDK1
Origin
Human
Theoretical MW
Affinity Tag
6His
Product Specifications
Expression system
Expressed by baculovirus in Sf21 insect cells
Purity
Refer to CoA for Purity
Purification method
Ni2+/NTA-agarose
Sample Buffer
Specified activity
Refer to CoA
Application
Storage conditions
6 months at -70°C
Usage disclaimer
For Research Only
Biological Information
Background
Phosphoinositide-Dependent Kinase-1 (PDK1) plays a central role in mediating cell signaling between PI3K and various intracellular serine/threonine kinases by phosphorylating many other kinases including PKC, p70S6 kinase, ribosomal S6 kinase, p21-activated kinase, PKC-related kinase, PKB and serum glucocorticoid kinase. PDK1 interacts with a wide range of substrates, and has been found to regulate apoptosis, cell proliferation and survival through the PI3K signaling pathway. A PDK1 inhibitor may be of therapeutic use in the treatment of human cancers and diabetes.
GenBank AF017995
Target class
Kinase
Family
AGC
Accession Number
NM_002613.3; NM_031268.4
Target Name
PDK1
Target Alias
PDPK1, MGC20087, PDK1, PkB-like, PRO0461, MGC35290, hPDK1
Origin
Human
Theoretical MW
Affinity Tag
6His
Product Specifications
Expression system
Expressed by baculovirus in Sf21 insect cells
Purity
Refer to CoA for Purity
Purification method
Ni2+/NTA-agarose
Sample Buffer
Specified activity
Refer to CoA
Application
Storage conditions
6 months at -70°C
Usage disclaimer
For Research Only