PDHK4, active Human Recombinant Kinase Protein
10 µg of active PDHK4. N-terminal GST-tagged, recombinant, human PDHK4 full length.
No items found.
Biological information
Background
Pyruvate dehydrogenase kinase isoenzyme 4 (PDHK4) is a member of the PDK/BCKDK protein kinase family. PDHK4 plays a key role in regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. PDHK4 is one of the three pyruvate dehydrogenase kinases that inhibits the PDH complex by phosphorylation of the E1 alpha subunit. This gene is predominantly expressed in the heart and skeletal muscles. [provided by RefSeq, Mar 2010]
Target class
Kinase
Family
Atypical-PDHK
Accession number
NM_002612.2
Target Name
PDHK4, active Human Recombinant Kinase Protein
Target Alias
PDK4; Pyruvate Dehydrogenase Kinase 4; Pyruvate Dehydrogenase Kinase, Isozyme 4
Origin
Human
Theori. MW
74 kDa
Affinity tag
GST
Product specifications
Expression system
Expressed by baculovirus in Sf21 insect cells
Purity
Purity 80% by SDS-PAGE and Coomassie blue staining
Purification method
glutathione agarose
Sample Buffer
enzyme in 50 mM Tris/HCl pH 8.0, 4 mM glutathione, 266 mM NaCl, 0.09 mM EGTA, 0.03% Brij-35, 270 mM sucrose, 0.9 mM benzamidine, 0.2 mM PMSF, 0.09% 2-mercaptoethanol. Frozen solution
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
1 year at -70°C
Usage disclaimer
For Research Only
Chemical data
Background
Pyruvate dehydrogenase kinase isoenzyme 4 (PDHK4) is a member of the PDK/BCKDK protein kinase family. PDHK4 plays a key role in regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. PDHK4 is one of the three pyruvate dehydrogenase kinases that inhibits the PDH complex by phosphorylation of the E1 alpha subunit. This gene is predominantly expressed in the heart and skeletal muscles. [provided by RefSeq, Mar 2010]
Compound name
Kinase
Catalog number
15-024
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Kinase Activity Assay Biological information
Background
Pyruvate dehydrogenase kinase isoenzyme 4 (PDHK4) is a member of the PDK/BCKDK protein kinase family. PDHK4 plays a key role in regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. PDHK4 is one of the three pyruvate dehydrogenase kinases that inhibits the PDH complex by phosphorylation of the E1 alpha subunit. This gene is predominantly expressed in the heart and skeletal muscles. [provided by RefSeq, Mar 2010]
Target class
Kinase
Family
Atypical-PDHK
Subfamily
Protein Name
PDHK4
Protein Alias
PDK4; Pyruvate Dehydrogenase Kinase 4; Pyruvate Dehydrogenase Kinase, Isozyme 4
Accession Number
NM_002612.2
UniProt Number
Gene Name
Gene ID
Gene Aliases
Target Species
Human
Kinase Activity Assay Usage
Product Type
Application
Storage Conditions
1 year at -70°C
Usage disclaimer
Kinase Activity Assay Information
Assay Type
Assay Measures
Biological Information
Background
Pyruvate dehydrogenase kinase isoenzyme 4 (PDHK4) is a member of the PDK/BCKDK protein kinase family. PDHK4 plays a key role in regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. PDHK4 is one of the three pyruvate dehydrogenase kinases that inhibits the PDH complex by phosphorylation of the E1 alpha subunit. This gene is predominantly expressed in the heart and skeletal muscles. [provided by RefSeq, Mar 2010]
Target class
Kinase
Family
Atypical-PDHK
Accession Number
NM_002612.2
Target Name
PDHK4
Target Alias
PDK4; Pyruvate Dehydrogenase Kinase 4; Pyruvate Dehydrogenase Kinase, Isozyme 4
Origin
Human
Theoretical MW
Affinity Tag
GST
Product Specifications
Expression system
Expressed by baculovirus in Sf21 insect cells
Purity
Purity 80% by SDS-PAGE and Coomassie blue staining
Purification method
glutathione agarose
Sample Buffer
Specified activity
Refer to CoA
Application
Storage conditions
1 year at -70°C
Usage disclaimer
For Research Only
Biological Information
Background
Pyruvate dehydrogenase kinase isoenzyme 4 (PDHK4) is a member of the PDK/BCKDK protein kinase family. PDHK4 plays a key role in regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. PDHK4 is one of the three pyruvate dehydrogenase kinases that inhibits the PDH complex by phosphorylation of the E1 alpha subunit. This gene is predominantly expressed in the heart and skeletal muscles. [provided by RefSeq, Mar 2010]
Target class
Kinase
Family
Atypical-PDHK
Accession Number
NM_002612.2
Target Name
PDHK4
Target Alias
PDK4; Pyruvate Dehydrogenase Kinase 4; Pyruvate Dehydrogenase Kinase, Isozyme 4
Origin
Human
Theoretical MW
Affinity Tag
GST
Product Specifications
Expression system
Expressed by baculovirus in Sf21 insect cells
Purity
Purity 80% by SDS-PAGE and Coomassie blue staining
Purification method
glutathione agarose
Sample Buffer
Specified activity
Refer to CoA
Application
Storage conditions
1 year at -70°C
Usage disclaimer
For Research Only