MAP4K4, active Human Recombinant Kinase Protein
N-terminal GST tagged and C-terminal 6His tagged, recombinant, human MAP4K4 amino acids 1-328, expressed by baculovirus in Sf21 insect cells. Purified using immobilized metal affinity chromatography.
No items found.
Biological information
Background
MAP4K4 is a member of the serine/threonine protein kinase family. This kinase has been shown to specifically activate MAPK8/JNK. The activation of MAPK8 by this kinase is found to be inhibited by the dominant-negative mutants of MAP3K7/TAK1, MAP2K4/MKK4, and MAP2K7/MKK7, which suggests that this kinase may function through the MAP3K7-MAP2K4-MAP2K7 kinase cascade, and mediate the TNF-alpha signaling pathway. Alternatively spliced transcript variants encoding different isoforms have been identified.
Target class
Kinase
Family
CMGC
Accession number
NM_004834.4
Target Name
MAP4K4, active Human Recombinant Kinase Protein
Target Alias
Origin
Human
Theori. MW
66 kDa
Affinity tag
GST
Product specifications
Expression system
Expressed by baculovirus in Sf21 insect cells
Purity
Purity 98% by SDS-PAGE and Coomassie blue staining.
Purification method
Immobilized metal affinity chromatography
Sample Buffer
3.20 mg/ml of enzyme in 50 mM Tris/HCl pH 7.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij-35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
1 year at -70°C
Usage disclaimer
For Research Only
Chemical data
Background
MAP4K4 is a member of the serine/threonine protein kinase family. This kinase has been shown to specifically activate MAPK8/JNK. The activation of MAPK8 by this kinase is found to be inhibited by the dominant-negative mutants of MAP3K7/TAK1, MAP2K4/MKK4, and MAP2K7/MKK7, which suggests that this kinase may function through the MAP3K7-MAP2K4-MAP2K7 kinase cascade, and mediate the TNF-alpha signaling pathway. Alternatively spliced transcript variants encoding different isoforms have been identified.
Compound name
Kinase
Catalog number
15-012
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Kinase Activity Assay Biological information
Background
MAP4K4 is a member of the serine/threonine protein kinase family. This kinase has been shown to specifically activate MAPK8/JNK. The activation of MAPK8 by this kinase is found to be inhibited by the dominant-negative mutants of MAP3K7/TAK1, MAP2K4/MKK4, and MAP2K7/MKK7, which suggests that this kinase may function through the MAP3K7-MAP2K4-MAP2K7 kinase cascade, and mediate the TNF-alpha signaling pathway. Alternatively spliced transcript variants encoding different isoforms have been identified.
Target class
Kinase
Family
CMGC
Subfamily
Protein Name
MAP4K4
Protein Alias
Accession Number
NM_004834.4
UniProt Number
Gene Name
Gene ID
Gene Aliases
Target Species
Human
Kinase Activity Assay Usage
Product Type
Application
Storage Conditions
1 year at -70°C
Usage disclaimer
Kinase Activity Assay Information
Assay Type
Assay Measures
Biological Information
Background
MAP4K4 is a member of the serine/threonine protein kinase family. This kinase has been shown to specifically activate MAPK8/JNK. The activation of MAPK8 by this kinase is found to be inhibited by the dominant-negative mutants of MAP3K7/TAK1, MAP2K4/MKK4, and MAP2K7/MKK7, which suggests that this kinase may function through the MAP3K7-MAP2K4-MAP2K7 kinase cascade, and mediate the TNF-alpha signaling pathway. Alternatively spliced transcript variants encoding different isoforms have been identified.
Target class
Kinase
Family
CMGC
Accession Number
NM_004834.4
Target Name
MAP4K4
Target Alias
Origin
Human
Theoretical MW
Affinity Tag
GST
Product Specifications
Expression system
Expressed by baculovirus in Sf21 insect cells
Purity
Purity 98% by SDS-PAGE and Coomassie blue staining.
Purification method
Immobilized metal affinity chromatography
Sample Buffer
Specified activity
Refer to CoA
Application
Storage conditions
1 year at -70°C
Usage disclaimer
For Research Only
Biological Information
Background
MAP4K4 is a member of the serine/threonine protein kinase family. This kinase has been shown to specifically activate MAPK8/JNK. The activation of MAPK8 by this kinase is found to be inhibited by the dominant-negative mutants of MAP3K7/TAK1, MAP2K4/MKK4, and MAP2K7/MKK7, which suggests that this kinase may function through the MAP3K7-MAP2K4-MAP2K7 kinase cascade, and mediate the TNF-alpha signaling pathway. Alternatively spliced transcript variants encoding different isoforms have been identified.
Target class
Kinase
Family
CMGC
Accession Number
NM_004834.4
Target Name
MAP4K4
Target Alias
Origin
Human
Theoretical MW
Affinity Tag
GST
Product Specifications
Expression system
Expressed by baculovirus in Sf21 insect cells
Purity
Purity 98% by SDS-PAGE and Coomassie blue staining.
Purification method
Immobilized metal affinity chromatography
Sample Buffer
Specified activity
Refer to CoA
Application
Storage conditions
1 year at -70°C
Usage disclaimer
For Research Only