Flt1, active Human Recombinant Kinase Protein
10 µg of active Flt1. N-terminal GST-tagged recombinant, human Flt1 amino acids 783-end.
No items found.
Biological information
Background
Two high affinity VEGF receptors, FLT1 (VEGFR-1, activated by placental growth factor PlGF) and KDR (Flk-1/VEGFR-2, activated by VEGF), have been identified. These receptors can be divided into three structural regions: seven extracellular Ig-like domains that contain the growth factor binding sites, a single polypeptide chain hydrophobic trans-membrane sequence, and intracellular cytoplasmic domains that confer the tyrosine kinase activity required for signal transduction. VEGF-B and VEGF-D homologues bind with high affinity to FLT1 (fms-related tyrosine kinase 1, vascular endothelial growth factor/vascular permeability factor receptor).
GenBank AF063657
Target class
Kinase
Family
TK
Accession number
AF063657
Target Name
Flt1, active Human Recombinant Kinase Protein
Target Alias
FLT, FLT-1, VEGFR1, VEGFR-1
Origin
Human
Theori. MW
89 kDa
Affinity tag
GST
Product specifications
Expression system
Expressed by baculovirus in Sf21 insect cells
Purity
Refer to CoA for Purity
Purification method
Glutathione Sepharose™ affinity chromatography
Sample Buffer
Protein in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
6 months at -70°C
Usage disclaimer
For Research Only
Chemical data
Background
Two high affinity VEGF receptors, FLT1 (VEGFR-1, activated by placental growth factor PlGF) and KDR (Flk-1/VEGFR-2, activated by VEGF), have been identified. These receptors can be divided into three structural regions: seven extracellular Ig-like domains that contain the growth factor binding sites, a single polypeptide chain hydrophobic trans-membrane sequence, and intracellular cytoplasmic domains that confer the tyrosine kinase activity required for signal transduction. VEGF-B and VEGF-D homologues bind with high affinity to FLT1 (fms-related tyrosine kinase 1, vascular endothelial growth factor/vascular permeability factor receptor).
GenBank AF063657
Compound name
Kinase
Catalog number
14-923
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Kinase Activity Assay Biological information
Background
Two high affinity VEGF receptors, FLT1 (VEGFR-1, activated by placental growth factor PlGF) and KDR (Flk-1/VEGFR-2, activated by VEGF), have been identified. These receptors can be divided into three structural regions: seven extracellular Ig-like domains that contain the growth factor binding sites, a single polypeptide chain hydrophobic trans-membrane sequence, and intracellular cytoplasmic domains that confer the tyrosine kinase activity required for signal transduction. VEGF-B and VEGF-D homologues bind with high affinity to FLT1 (fms-related tyrosine kinase 1, vascular endothelial growth factor/vascular permeability factor receptor).
GenBank AF063657
Target class
Kinase
Family
TK
Subfamily
Protein Name
Flt1
Protein Alias
FLT, FLT-1, VEGFR1, VEGFR-1
Accession Number
AF063657
UniProt Number
Gene Name
Gene ID
Gene Aliases
Target Species
Human
Kinase Activity Assay Usage
Product Type
Application
Storage Conditions
6 months at -70°C
Usage disclaimer
Kinase Activity Assay Information
Assay Type
Assay Measures
Biological Information
Background
Two high affinity VEGF receptors, FLT1 (VEGFR-1, activated by placental growth factor PlGF) and KDR (Flk-1/VEGFR-2, activated by VEGF), have been identified. These receptors can be divided into three structural regions: seven extracellular Ig-like domains that contain the growth factor binding sites, a single polypeptide chain hydrophobic trans-membrane sequence, and intracellular cytoplasmic domains that confer the tyrosine kinase activity required for signal transduction. VEGF-B and VEGF-D homologues bind with high affinity to FLT1 (fms-related tyrosine kinase 1, vascular endothelial growth factor/vascular permeability factor receptor).
GenBank AF063657
Target class
Kinase
Family
TK
Accession Number
AF063657
Target Name
Flt1
Target Alias
FLT, FLT-1, VEGFR1, VEGFR-1
Origin
Human
Theoretical MW
Affinity Tag
GST
Product Specifications
Expression system
Expressed by baculovirus in Sf21 insect cells
Purity
Refer to CoA for Purity
Purification method
Glutathione Sepharose™ affinity chromatography
Sample Buffer
Specified activity
Refer to CoA
Application
Storage conditions
6 months at -70°C
Usage disclaimer
For Research Only
Biological Information
Background
Two high affinity VEGF receptors, FLT1 (VEGFR-1, activated by placental growth factor PlGF) and KDR (Flk-1/VEGFR-2, activated by VEGF), have been identified. These receptors can be divided into three structural regions: seven extracellular Ig-like domains that contain the growth factor binding sites, a single polypeptide chain hydrophobic trans-membrane sequence, and intracellular cytoplasmic domains that confer the tyrosine kinase activity required for signal transduction. VEGF-B and VEGF-D homologues bind with high affinity to FLT1 (fms-related tyrosine kinase 1, vascular endothelial growth factor/vascular permeability factor receptor).
GenBank AF063657
Target class
Kinase
Family
TK
Accession Number
AF063657
Target Name
Flt1
Target Alias
FLT, FLT-1, VEGFR1, VEGFR-1
Origin
Human
Theoretical MW
Affinity Tag
GST
Product Specifications
Expression system
Expressed by baculovirus in Sf21 insect cells
Purity
Refer to CoA for Purity
Purification method
Glutathione Sepharose™ affinity chromatography
Sample Buffer
Specified activity
Refer to CoA
Application
Storage conditions
6 months at -70°C
Usage disclaimer
For Research Only