DAPK2, active Human Recombinant Kinase Protein
N-terminal GST-tagged recombinant, human full length DAPK2.
No items found.
Biological information
Background
Death-associated protein kinase (DAPK) is a calcium/calmodulin-dependent kinase that phosphorylates serine/threonine residues on proteins. DAPK modulates cell death induced by IFN-g, TNF-a, Fas, and detachment from the extracellular matrix. DAPK has been also implicated in TGF-binduced apoptosis in several cultured cell lines in which Smad proteins mediate transcriptional activation of DAPK. A member of DAP kinase family, DAPK2 is a 42 KDa calcium/calmodulin-dependant serine/threonine kinase localized in the cytoplasm. Reports suggest DAPK2 along with DAPK1 induces membrane blebbing and formation of autophagic vesicles and plays a vital role in TGF-induced caspase-dependent Type-I and IFNg-induced caspase-independent autophagic type II cell death signaling. Inhibition of DAPK2 may facilitate tumor progression.
GenBank NM_014326
Target class
Kinase
Family
CAMK
Accession number
NM_014326.3
Target Name
DAPK2, active Human Recombinant Kinase Protein
Target Alias
DAPK2, DRP-1, MGC119312
Origin
Human
Theori. MW
70.6 kDa
Affinity tag
GST
Product specifications
Expression system
Expressed by baculovirus in Sf21 insect cells
Purity
Refer to CoA for Purity
Purification method
Ni2+/NTA-agarose
Sample Buffer
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
6 months at -70°C
Usage disclaimer
For Research Only
Chemical data
Background
Death-associated protein kinase (DAPK) is a calcium/calmodulin-dependent kinase that phosphorylates serine/threonine residues on proteins. DAPK modulates cell death induced by IFN-g, TNF-a, Fas, and detachment from the extracellular matrix. DAPK has been also implicated in TGF-binduced apoptosis in several cultured cell lines in which Smad proteins mediate transcriptional activation of DAPK. A member of DAP kinase family, DAPK2 is a 42 KDa calcium/calmodulin-dependant serine/threonine kinase localized in the cytoplasm. Reports suggest DAPK2 along with DAPK1 induces membrane blebbing and formation of autophagic vesicles and plays a vital role in TGF-induced caspase-dependent Type-I and IFNg-induced caspase-independent autophagic type II cell death signaling. Inhibition of DAPK2 may facilitate tumor progression.
GenBank NM_014326
Compound name
Kinase
Catalog number
14-657
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Lipid Kinase Activity Assay Biological information
Background
Death-associated protein kinase (DAPK) is a calcium/calmodulin-dependent kinase that phosphorylates serine/threonine residues on proteins. DAPK modulates cell death induced by IFN-g, TNF-a, Fas, and detachment from the extracellular matrix. DAPK has been also implicated in TGF-binduced apoptosis in several cultured cell lines in which Smad proteins mediate transcriptional activation of DAPK. A member of DAP kinase family, DAPK2 is a 42 KDa calcium/calmodulin-dependant serine/threonine kinase localized in the cytoplasm. Reports suggest DAPK2 along with DAPK1 induces membrane blebbing and formation of autophagic vesicles and plays a vital role in TGF-induced caspase-dependent Type-I and IFNg-induced caspase-independent autophagic type II cell death signaling. Inhibition of DAPK2 may facilitate tumor progression.
GenBank NM_014326
Target class
Kinase
Family
CAMK
Subfamily
Protein Name
DAPK2
Protein Alias
DAPK2, DRP-1, MGC119312
Accession Number
NM_014326.3
UniProt Number
Gene Name
Gene ID
Gene Aliases
Target Species
Human
Lipid Kinase Activity Assay Usage
Product Type
Application
Storage Conditions
6 months at -70°C
Usage disclaimer
Lipid Kinase Activity Assay Information
Assay Type
Assay Measures
Biological Information
Background
Death-associated protein kinase (DAPK) is a calcium/calmodulin-dependent kinase that phosphorylates serine/threonine residues on proteins. DAPK modulates cell death induced by IFN-g, TNF-a, Fas, and detachment from the extracellular matrix. DAPK has been also implicated in TGF-binduced apoptosis in several cultured cell lines in which Smad proteins mediate transcriptional activation of DAPK. A member of DAP kinase family, DAPK2 is a 42 KDa calcium/calmodulin-dependant serine/threonine kinase localized in the cytoplasm. Reports suggest DAPK2 along with DAPK1 induces membrane blebbing and formation of autophagic vesicles and plays a vital role in TGF-induced caspase-dependent Type-I and IFNg-induced caspase-independent autophagic type II cell death signaling. Inhibition of DAPK2 may facilitate tumor progression.
GenBank NM_014326
Target class
Kinase
Family
CAMK
Accession Number
NM_014326.3
Target Name
DAPK2
Target Alias
DAPK2, DRP-1, MGC119312
Origin
Human
Theoretical MW
Affinity Tag
GST
Product Specifications
Expression system
Expressed by baculovirus in Sf21 insect cells
Purity
Refer to CoA for Purity
Purification method
Ni2+/NTA-agarose
Sample Buffer
Specified activity
Refer to CoA
Application
Storage conditions
6 months at -70°C
Usage disclaimer
For Research Only
Biological Information
Background
Death-associated protein kinase (DAPK) is a calcium/calmodulin-dependent kinase that phosphorylates serine/threonine residues on proteins. DAPK modulates cell death induced by IFN-g, TNF-a, Fas, and detachment from the extracellular matrix. DAPK has been also implicated in TGF-binduced apoptosis in several cultured cell lines in which Smad proteins mediate transcriptional activation of DAPK. A member of DAP kinase family, DAPK2 is a 42 KDa calcium/calmodulin-dependant serine/threonine kinase localized in the cytoplasm. Reports suggest DAPK2 along with DAPK1 induces membrane blebbing and formation of autophagic vesicles and plays a vital role in TGF-induced caspase-dependent Type-I and IFNg-induced caspase-independent autophagic type II cell death signaling. Inhibition of DAPK2 may facilitate tumor progression.
GenBank NM_014326
Target class
Kinase
Family
CAMK
Accession Number
NM_014326.3
Target Name
DAPK2
Target Alias
DAPK2, DRP-1, MGC119312
Origin
Human
Theoretical MW
Affinity Tag
GST
Product Specifications
Expression system
Expressed by baculovirus in Sf21 insect cells
Purity
Refer to CoA for Purity
Purification method
Ni2+/NTA-agarose
Sample Buffer
Specified activity
Refer to CoA
Application
Storage conditions
6 months at -70°C
Usage disclaimer
For Research Only