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Syk Kinase Enzyme Activity Assay Kit

The Syk Kinase Enzyme Activity Assay Kit offers a validated, ready-to-use biochemical assay based on the renowned ADP Hunter™ Plus Assay platform from DiscoverX for accurately measuring kinase inhibitor efficacy. The kit includes recombinant Syk, substrate, ATP, detection reagents, and assay plates, enabling precise quantification of ADP accumulation. Designed to handle elevated ATP concentrations, this assay delivers robust, reliable data ideal for high-throughput screening (HTS) and other drug discovery applications.

Biological information

Background

The Kinase Enzyme Activity Assay Kit provides a reliable biochemical method for evaluating kinase inhibitors. Built on HitHunter® assay technology, this kit is specifically optimized for Syk. It comes with all essential components, including recombinant Syk (Eurofins #14-314), a substrate, ATP, detection reagents, and assay plates. Each kit is accompanied by a Certificate of Analysis (COA), which outlines the recommended reaction conditions for accurate inhibitor detection. The supplied materials are sufficient to perform experiments covering two, six or twenty five 384-well assay plates.

Spleen tyrosine kinase (Syk) is a non-receptor tyrosine kinase involved in immune cell signaling, particularly downstream of the B-cell receptor (BCR) and Fc receptor pathways. Also referred to as p72-Syk, this kinase regulates inflammation, autoimmune responses, and tumor progression. Because of its strong link to hematological cancers and immune-mediated diseases, Syk is a high-value target in drug discovery and kinase inhibitor research. A Syk enzyme activity assay kit enables detailed characterization of Syk inhibitors, supporting the development of therapeutics against cancer and autoimmune disorders.

Target class
Kinase
Family
SYK/ZAP-70
Accession number
GenBank L28824.1
Target Name
Syk Kinase Enzyme Activity Assay Kit
Target Alias
p72-Syk
Origin
Human
Theori. MW
Affinity tag

Product specifications

Expression system
Purity
Purification method
Sample Buffer
Specified activity
Application
Storage conditions
1 year at -70°C
Usage disclaimer

Chemical data

Background

The Kinase Enzyme Activity Assay Kit provides a reliable biochemical method for evaluating kinase inhibitors. Built on HitHunter® assay technology, this kit is specifically optimized for Syk. It comes with all essential components, including recombinant Syk (Eurofins #14-314), a substrate, ATP, detection reagents, and assay plates. Each kit is accompanied by a Certificate of Analysis (COA), which outlines the recommended reaction conditions for accurate inhibitor detection. The supplied materials are sufficient to perform experiments covering two, six or twenty five 384-well assay plates.

Spleen tyrosine kinase (Syk) is a non-receptor tyrosine kinase involved in immune cell signaling, particularly downstream of the B-cell receptor (BCR) and Fc receptor pathways. Also referred to as p72-Syk, this kinase regulates inflammation, autoimmune responses, and tumor progression. Because of its strong link to hematological cancers and immune-mediated diseases, Syk is a high-value target in drug discovery and kinase inhibitor research. A Syk enzyme activity assay kit enables detailed characterization of Syk inhibitors, supporting the development of therapeutics against cancer and autoimmune disorders.

Compound name
Kinase
Catalog number
44-314
Molecular formula
CAS
MW
Ka
Percent composition

Product specifications

Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
Solubility structure

Kinase Activity Assay Biological information

Background

The Kinase Enzyme Activity Assay Kit provides a reliable biochemical method for evaluating kinase inhibitors. Built on HitHunter® assay technology, this kit is specifically optimized for Syk. It comes with all essential components, including recombinant Syk (Eurofins #14-314), a substrate, ATP, detection reagents, and assay plates. Each kit is accompanied by a Certificate of Analysis (COA), which outlines the recommended reaction conditions for accurate inhibitor detection. The supplied materials are sufficient to perform experiments covering two, six or twenty five 384-well assay plates.

Spleen tyrosine kinase (Syk) is a non-receptor tyrosine kinase involved in immune cell signaling, particularly downstream of the B-cell receptor (BCR) and Fc receptor pathways. Also referred to as p72-Syk, this kinase regulates inflammation, autoimmune responses, and tumor progression. Because of its strong link to hematological cancers and immune-mediated diseases, Syk is a high-value target in drug discovery and kinase inhibitor research. A Syk enzyme activity assay kit enables detailed characterization of Syk inhibitors, supporting the development of therapeutics against cancer and autoimmune disorders.

Target class
Kinase
Family
SYK/ZAP-70
Subfamily
Tyrosine kinase
Protein Name
Syk
Protein Alias
p72-Syk
Accession Number
GenBank L28824.1
UniProt Number
P43405
Gene Name
SYK
Gene ID
6850
Gene Aliases

IMD82; p72-Syk

Target Species
Human

Kinase Activity Assay Usage

Product Type
Enzyme Activity Assay Kits
Application
Drug Discovery & Development
Storage Conditions
1 year at -70°C
Usage disclaimer

For Research Use Only. These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.

Kinase Activity Assay Information

Assay Type
Enzymatic
Assay Measures
ADP Accumulation

Biological Information

Background

The Kinase Enzyme Activity Assay Kit provides a reliable biochemical method for evaluating kinase inhibitors. Built on HitHunter® assay technology, this kit is specifically optimized for Syk. It comes with all essential components, including recombinant Syk (Eurofins #14-314), a substrate, ATP, detection reagents, and assay plates. Each kit is accompanied by a Certificate of Analysis (COA), which outlines the recommended reaction conditions for accurate inhibitor detection. The supplied materials are sufficient to perform experiments covering two, six or twenty five 384-well assay plates.

Spleen tyrosine kinase (Syk) is a non-receptor tyrosine kinase involved in immune cell signaling, particularly downstream of the B-cell receptor (BCR) and Fc receptor pathways. Also referred to as p72-Syk, this kinase regulates inflammation, autoimmune responses, and tumor progression. Because of its strong link to hematological cancers and immune-mediated diseases, Syk is a high-value target in drug discovery and kinase inhibitor research. A Syk enzyme activity assay kit enables detailed characterization of Syk inhibitors, supporting the development of therapeutics against cancer and autoimmune disorders.

Target class
Kinase
Family
SYK/ZAP-70
Accession Number
GenBank L28824.1
Target Name
Syk
Target Alias
p72-Syk
Origin
Human
Theoretical MW
Affinity Tag

Product Specifications

Expression system
Purity
Purification method
Sample Buffer
Enzymatic
Specified activity
Application
Drug Discovery & Development
Storage conditions
1 year at -70°C
Usage disclaimer

For Research Use Only. These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.

Biological Information

Background

The Kinase Enzyme Activity Assay Kit provides a reliable biochemical method for evaluating kinase inhibitors. Built on HitHunter® assay technology, this kit is specifically optimized for Syk. It comes with all essential components, including recombinant Syk (Eurofins #14-314), a substrate, ATP, detection reagents, and assay plates. Each kit is accompanied by a Certificate of Analysis (COA), which outlines the recommended reaction conditions for accurate inhibitor detection. The supplied materials are sufficient to perform experiments covering two, six or twenty five 384-well assay plates.

Spleen tyrosine kinase (Syk) is a non-receptor tyrosine kinase involved in immune cell signaling, particularly downstream of the B-cell receptor (BCR) and Fc receptor pathways. Also referred to as p72-Syk, this kinase regulates inflammation, autoimmune responses, and tumor progression. Because of its strong link to hematological cancers and immune-mediated diseases, Syk is a high-value target in drug discovery and kinase inhibitor research. A Syk enzyme activity assay kit enables detailed characterization of Syk inhibitors, supporting the development of therapeutics against cancer and autoimmune disorders.

Target class
Kinase
Family
SYK/ZAP-70
Accession Number
GenBank L28824.1
Target Name
Syk
Target Alias
p72-Syk
Origin
Human
Theoretical MW
Affinity Tag

Product Specifications

Expression system
Purity
Purification method
Sample Buffer
Enzymatic
Specified activity
Application
Drug Discovery & Development
Storage conditions
1 year at -70°C
Usage disclaimer

For Research Use Only. These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.

Datasheets
44-314x Datasheet
User Manual
File N°2
Kinase Enzyme Activity Assay User Manual Rev 1
€ 950,00 EUR
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