Brought to you by:

SRC Kinase Enzyme Activity Assay Kit

The SRC Kinase Enzyme Activity Assay Kit offers a validated, ready-to-use biochemical assay based on the renowned ADP Hunter™ Plus Assay platform from DiscoverX for accurately measuring kinase inhibitor efficacy. The kit includes recombinant SRC, substrate, ATP, detection reagents, and assay plates, enabling precise quantification of ADP accumulation. Designed to handle elevated ATP concentrations, this assay delivers robust, reliable data ideal for high-throughput screening (HTS) and other drug discovery applications.

Biological information

Background

The Kinase Enzyme Activity Assay Kit provides a reliable biochemical method for evaluating kinase inhibitors. Built on HitHunter® assay technology, this kit is specifically optimized for Src. It comes with all essential components, including recombinant Src (Eurofins #14-326), a substrate, ATP, detection reagents, and assay plates. Each kit is accompanied by a Certificate of Analysis (COA), which outlines the recommended reaction conditions for accurate inhibitor detection. The supplied materials are sufficient to perform experiments covering two, six or twenty five 384-well assay plates.

The proto-oncogene tyrosine-protein kinase SRC (also known as pp60c-src) is a founding member of the Src family kinases (SFKs). SRC regulates cell adhesion, proliferation, migration, and survival. Aberrant activation of SRC signaling contributes to breast, colon, lung, and pancreatic cancers, making it a major focus in oncology drug discovery. A SRC enzyme activity assay kit provides a robust platform for studying inhibitor potency and specificity, helping researchers design targeted therapies against SRC-driven tumorigenesis.

Target class
Kinase
Family
SRC
Accession number
GenBank NM_005417.5
Target Name
SRC Kinase Enzyme Activity Assay Kit
Target Alias
c-Src; pp60c-src; p60-Src
Origin
Human
Theori. MW
Affinity tag

Product specifications

Expression system
Purity
Purification method
Sample Buffer
Specified activity
Application
Storage conditions
1 year at -70°C
Usage disclaimer

Chemical data

Background

The Kinase Enzyme Activity Assay Kit provides a reliable biochemical method for evaluating kinase inhibitors. Built on HitHunter® assay technology, this kit is specifically optimized for Src. It comes with all essential components, including recombinant Src (Eurofins #14-326), a substrate, ATP, detection reagents, and assay plates. Each kit is accompanied by a Certificate of Analysis (COA), which outlines the recommended reaction conditions for accurate inhibitor detection. The supplied materials are sufficient to perform experiments covering two, six or twenty five 384-well assay plates.

The proto-oncogene tyrosine-protein kinase SRC (also known as pp60c-src) is a founding member of the Src family kinases (SFKs). SRC regulates cell adhesion, proliferation, migration, and survival. Aberrant activation of SRC signaling contributes to breast, colon, lung, and pancreatic cancers, making it a major focus in oncology drug discovery. A SRC enzyme activity assay kit provides a robust platform for studying inhibitor potency and specificity, helping researchers design targeted therapies against SRC-driven tumorigenesis.

Compound name
Kinase
Catalog number
44-326
Molecular formula
CAS
MW
Ka
Percent composition

Product specifications

Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
Solubility structure

Kinase Activity Assay Biological information

Background

The Kinase Enzyme Activity Assay Kit provides a reliable biochemical method for evaluating kinase inhibitors. Built on HitHunter® assay technology, this kit is specifically optimized for Src. It comes with all essential components, including recombinant Src (Eurofins #14-326), a substrate, ATP, detection reagents, and assay plates. Each kit is accompanied by a Certificate of Analysis (COA), which outlines the recommended reaction conditions for accurate inhibitor detection. The supplied materials are sufficient to perform experiments covering two, six or twenty five 384-well assay plates.

The proto-oncogene tyrosine-protein kinase SRC (also known as pp60c-src) is a founding member of the Src family kinases (SFKs). SRC regulates cell adhesion, proliferation, migration, and survival. Aberrant activation of SRC signaling contributes to breast, colon, lung, and pancreatic cancers, making it a major focus in oncology drug discovery. A SRC enzyme activity assay kit provides a robust platform for studying inhibitor potency and specificity, helping researchers design targeted therapies against SRC-driven tumorigenesis.

Target class
Kinase
Family
SRC
Subfamily
Tyrosine kinase (non-receptor)
Protein Name
Src
Protein Alias
c-Src; pp60c-src; p60-Src
Accession Number
GenBank NM_005417.5
UniProt Number
P12931
Gene Name
SRC
Gene ID
6714
Gene Aliases

ASV; SRC1; THC6; c-SRC; p60-Src

Target Species
Human

Kinase Activity Assay Usage

Product Type
Enzyme Activity Assay Kits
Application
Drug Discovery & Development
Storage Conditions
1 year at -70°C
Usage disclaimer

For Research Use Only. These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.

Kinase Activity Assay Information

Assay Type
Enzymatic
Assay Measures
ADP Accumulation

Biological Information

Background

The Kinase Enzyme Activity Assay Kit provides a reliable biochemical method for evaluating kinase inhibitors. Built on HitHunter® assay technology, this kit is specifically optimized for Src. It comes with all essential components, including recombinant Src (Eurofins #14-326), a substrate, ATP, detection reagents, and assay plates. Each kit is accompanied by a Certificate of Analysis (COA), which outlines the recommended reaction conditions for accurate inhibitor detection. The supplied materials are sufficient to perform experiments covering two, six or twenty five 384-well assay plates.

The proto-oncogene tyrosine-protein kinase SRC (also known as pp60c-src) is a founding member of the Src family kinases (SFKs). SRC regulates cell adhesion, proliferation, migration, and survival. Aberrant activation of SRC signaling contributes to breast, colon, lung, and pancreatic cancers, making it a major focus in oncology drug discovery. A SRC enzyme activity assay kit provides a robust platform for studying inhibitor potency and specificity, helping researchers design targeted therapies against SRC-driven tumorigenesis.

Target class
Kinase
Family
SRC
Accession Number
GenBank NM_005417.5
Target Name
Src
Target Alias
c-Src; pp60c-src; p60-Src
Origin
Human
Theoretical MW
Affinity Tag

Product Specifications

Expression system
Purity
Purification method
Sample Buffer
Enzymatic
Specified activity
Application
Drug Discovery & Development
Storage conditions
1 year at -70°C
Usage disclaimer

For Research Use Only. These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.

Biological Information

Background

The Kinase Enzyme Activity Assay Kit provides a reliable biochemical method for evaluating kinase inhibitors. Built on HitHunter® assay technology, this kit is specifically optimized for Src. It comes with all essential components, including recombinant Src (Eurofins #14-326), a substrate, ATP, detection reagents, and assay plates. Each kit is accompanied by a Certificate of Analysis (COA), which outlines the recommended reaction conditions for accurate inhibitor detection. The supplied materials are sufficient to perform experiments covering two, six or twenty five 384-well assay plates.

The proto-oncogene tyrosine-protein kinase SRC (also known as pp60c-src) is a founding member of the Src family kinases (SFKs). SRC regulates cell adhesion, proliferation, migration, and survival. Aberrant activation of SRC signaling contributes to breast, colon, lung, and pancreatic cancers, making it a major focus in oncology drug discovery. A SRC enzyme activity assay kit provides a robust platform for studying inhibitor potency and specificity, helping researchers design targeted therapies against SRC-driven tumorigenesis.

Target class
Kinase
Family
SRC
Accession Number
GenBank NM_005417.5
Target Name
Src
Target Alias
c-Src; pp60c-src; p60-Src
Origin
Human
Theoretical MW
Affinity Tag

Product Specifications

Expression system
Purity
Purification method
Sample Buffer
Enzymatic
Specified activity
Application
Drug Discovery & Development
Storage conditions
1 year at -70°C
Usage disclaimer

For Research Use Only. These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.

Datasheets
44-326x Datasheet
User Manual
File N°2
Kinase Enzyme Activity Assay User Manual Rev 1
€ 950,00 EUR
Purchase from our distributor
Button