SGK1 (delta1-59, S422D) Kinase Enzyme Activity Assay Kit
The SGK1 Kinase Enzyme Activity Assay Kit offers a validated, ready-to-use biochemical assay based on the renowned ADP Hunter™ Plus Assay platform from DiscoverX for accurately measuring kinase inhibitor efficacy. The kit includes recombinant SGK1, substrate, ATP, detection reagents, and assay plates, enabling precise quantification of ADP accumulation. Designed to handle elevated ATP concentrations, this assay delivers robust, reliable data ideal for high-throughput screening (HTS) and other drug discovery applications.
Biological information
The Kinase Enzyme Activity Assay Kit provides a reliable biochemical method for evaluating kinase inhibitors. Built on HitHunter® assay technology, this kit is specifically optimized for SGK1. It comes with all essential components, including recombinant SGK1 (Eurofins #14-331), a substrate, ATP, detection reagents, and assay plates. Each kit is accompanied by a Certificate of Analysis (COA), which outlines the recommended reaction conditions for accurate inhibitor detection. The supplied materials are sufficient to perform experiments covering two, six or twenty five 384-well assay plates.
SGK1 is a serine/threonine protein kinase that is transcriptionally regulated by serum and glucocorticoids and is activated downstream of phosphatidylinositol 3-kinase (PI3K) via phosphorylation by PDK1 and mTORC2. The kinase regulates diverse cellular processes including cell proliferation, survival, apoptosis, ion transport and cell volume regulation and shares many substrates with AKT, thereby acting as a parallel effector of PI3K signalling. SGK1 has a prominent role in epithelial physiology as well as immune regulation and inflammation. Dysregulated SGK1 expression/activity has been implicated in hypertension, fibrosis, autoimmune disease, cancer and neurodegenerative disorders, and as such SGK1 has emerged as a potential therapeutic target across multiple pathological conditions.
Product specifications
Chemical data
The Kinase Enzyme Activity Assay Kit provides a reliable biochemical method for evaluating kinase inhibitors. Built on HitHunter® assay technology, this kit is specifically optimized for SGK1. It comes with all essential components, including recombinant SGK1 (Eurofins #14-331), a substrate, ATP, detection reagents, and assay plates. Each kit is accompanied by a Certificate of Analysis (COA), which outlines the recommended reaction conditions for accurate inhibitor detection. The supplied materials are sufficient to perform experiments covering two, six or twenty five 384-well assay plates.
SGK1 is a serine/threonine protein kinase that is transcriptionally regulated by serum and glucocorticoids and is activated downstream of phosphatidylinositol 3-kinase (PI3K) via phosphorylation by PDK1 and mTORC2. The kinase regulates diverse cellular processes including cell proliferation, survival, apoptosis, ion transport and cell volume regulation and shares many substrates with AKT, thereby acting as a parallel effector of PI3K signalling. SGK1 has a prominent role in epithelial physiology as well as immune regulation and inflammation. Dysregulated SGK1 expression/activity has been implicated in hypertension, fibrosis, autoimmune disease, cancer and neurodegenerative disorders, and as such SGK1 has emerged as a potential therapeutic target across multiple pathological conditions.
Product specifications
Kinase Activity Assay Biological information
The Kinase Enzyme Activity Assay Kit provides a reliable biochemical method for evaluating kinase inhibitors. Built on HitHunter® assay technology, this kit is specifically optimized for SGK1. It comes with all essential components, including recombinant SGK1 (Eurofins #14-331), a substrate, ATP, detection reagents, and assay plates. Each kit is accompanied by a Certificate of Analysis (COA), which outlines the recommended reaction conditions for accurate inhibitor detection. The supplied materials are sufficient to perform experiments covering two, six or twenty five 384-well assay plates.
SGK1 is a serine/threonine protein kinase that is transcriptionally regulated by serum and glucocorticoids and is activated downstream of phosphatidylinositol 3-kinase (PI3K) via phosphorylation by PDK1 and mTORC2. The kinase regulates diverse cellular processes including cell proliferation, survival, apoptosis, ion transport and cell volume regulation and shares many substrates with AKT, thereby acting as a parallel effector of PI3K signalling. SGK1 has a prominent role in epithelial physiology as well as immune regulation and inflammation. Dysregulated SGK1 expression/activity has been implicated in hypertension, fibrosis, autoimmune disease, cancer and neurodegenerative disorders, and as such SGK1 has emerged as a potential therapeutic target across multiple pathological conditions.
SGK
Kinase Activity Assay Usage
For Research Use Only
Kinase Activity Assay Information
Biological Information
The Kinase Enzyme Activity Assay Kit provides a reliable biochemical method for evaluating kinase inhibitors. Built on HitHunter® assay technology, this kit is specifically optimized for SGK1. It comes with all essential components, including recombinant SGK1 (Eurofins #14-331), a substrate, ATP, detection reagents, and assay plates. Each kit is accompanied by a Certificate of Analysis (COA), which outlines the recommended reaction conditions for accurate inhibitor detection. The supplied materials are sufficient to perform experiments covering two, six or twenty five 384-well assay plates.
SGK1 is a serine/threonine protein kinase that is transcriptionally regulated by serum and glucocorticoids and is activated downstream of phosphatidylinositol 3-kinase (PI3K) via phosphorylation by PDK1 and mTORC2. The kinase regulates diverse cellular processes including cell proliferation, survival, apoptosis, ion transport and cell volume regulation and shares many substrates with AKT, thereby acting as a parallel effector of PI3K signalling. SGK1 has a prominent role in epithelial physiology as well as immune regulation and inflammation. Dysregulated SGK1 expression/activity has been implicated in hypertension, fibrosis, autoimmune disease, cancer and neurodegenerative disorders, and as such SGK1 has emerged as a potential therapeutic target across multiple pathological conditions.
Product Specifications
For Research Use Only
Biological Information
The Kinase Enzyme Activity Assay Kit provides a reliable biochemical method for evaluating kinase inhibitors. Built on HitHunter® assay technology, this kit is specifically optimized for SGK1. It comes with all essential components, including recombinant SGK1 (Eurofins #14-331), a substrate, ATP, detection reagents, and assay plates. Each kit is accompanied by a Certificate of Analysis (COA), which outlines the recommended reaction conditions for accurate inhibitor detection. The supplied materials are sufficient to perform experiments covering two, six or twenty five 384-well assay plates.
SGK1 is a serine/threonine protein kinase that is transcriptionally regulated by serum and glucocorticoids and is activated downstream of phosphatidylinositol 3-kinase (PI3K) via phosphorylation by PDK1 and mTORC2. The kinase regulates diverse cellular processes including cell proliferation, survival, apoptosis, ion transport and cell volume regulation and shares many substrates with AKT, thereby acting as a parallel effector of PI3K signalling. SGK1 has a prominent role in epithelial physiology as well as immune regulation and inflammation. Dysregulated SGK1 expression/activity has been implicated in hypertension, fibrosis, autoimmune disease, cancer and neurodegenerative disorders, and as such SGK1 has emerged as a potential therapeutic target across multiple pathological conditions.
Product Specifications
For Research Use Only