Akt2/PKBbeta (deltaPH, S474D) Kinase Enzyme Activity Assay Kit
The AKT2 Kinase Enzyme Activity Assay Kit offers a validated, ready-to-use biochemical assay based on the renowned ADP Hunter™ Plus Assay platform from DiscoverX for accurately measuring kinase inhibitor efficacy. The kit includes recombinant PKB beta, substrate, ATP, detection reagents, and assay plates, enabling precise quantification of ADP accumulation. Designed to handle elevated ATP concentrations, this assay delivers robust, reliable data ideal for high-throughput screening (HTS) and other drug discovery applications.
Biological information
The Kinase Enzyme Activity Assay Kit provides a reliable biochemical method for evaluating kinase inhibitors. Built on HitHunter® assay technology, this kit is specifically optimized for AKT2. It comes with all essential components, including recombinant AKT2 (Eurofins #14-339), a substrate, ATP, detection reagents, and assay plates. Each kit is accompanied by a Certificate of Analysis (COA), which outlines the recommended reaction conditions for accurate inhibitor detection. The supplied materials are sufficient to perform experiments covering two, six or twenty five 384-well assay plates.
AKT2 is a serine/threonine kinase and a member of the AKT family, alongside AKT1 and AKT3, collectively referred to as AKT kinase. AKT2 functions as a central node in cellular signalling downstream of PI3K, mediating responses to growth factors such as insulin, IGF1, PDGF, and EGF. It regulates diverse cellular processes including metabolism, proliferation, survival, growth, angiogenesis, cell migration, and differentiation through phosphorylation of multiple downstream substrates.
AKT2 contributes to both metabolic disorders and cancer. AKT2 activity is linked to insulin signalling defects and diabetic complications, while in oncology the kinase has been shown to drive tumour growth and metastasis.
Product specifications
Chemical data
The Kinase Enzyme Activity Assay Kit provides a reliable biochemical method for evaluating kinase inhibitors. Built on HitHunter® assay technology, this kit is specifically optimized for AKT2. It comes with all essential components, including recombinant AKT2 (Eurofins #14-339), a substrate, ATP, detection reagents, and assay plates. Each kit is accompanied by a Certificate of Analysis (COA), which outlines the recommended reaction conditions for accurate inhibitor detection. The supplied materials are sufficient to perform experiments covering two, six or twenty five 384-well assay plates.
AKT2 is a serine/threonine kinase and a member of the AKT family, alongside AKT1 and AKT3, collectively referred to as AKT kinase. AKT2 functions as a central node in cellular signalling downstream of PI3K, mediating responses to growth factors such as insulin, IGF1, PDGF, and EGF. It regulates diverse cellular processes including metabolism, proliferation, survival, growth, angiogenesis, cell migration, and differentiation through phosphorylation of multiple downstream substrates.
AKT2 contributes to both metabolic disorders and cancer. AKT2 activity is linked to insulin signalling defects and diabetic complications, while in oncology the kinase has been shown to drive tumour growth and metastasis.
Product specifications
Kinase Activity Assay Biological information
The Kinase Enzyme Activity Assay Kit provides a reliable biochemical method for evaluating kinase inhibitors. Built on HitHunter® assay technology, this kit is specifically optimized for AKT2. It comes with all essential components, including recombinant AKT2 (Eurofins #14-339), a substrate, ATP, detection reagents, and assay plates. Each kit is accompanied by a Certificate of Analysis (COA), which outlines the recommended reaction conditions for accurate inhibitor detection. The supplied materials are sufficient to perform experiments covering two, six or twenty five 384-well assay plates.
AKT2 is a serine/threonine kinase and a member of the AKT family, alongside AKT1 and AKT3, collectively referred to as AKT kinase. AKT2 functions as a central node in cellular signalling downstream of PI3K, mediating responses to growth factors such as insulin, IGF1, PDGF, and EGF. It regulates diverse cellular processes including metabolism, proliferation, survival, growth, angiogenesis, cell migration, and differentiation through phosphorylation of multiple downstream substrates.
AKT2 contributes to both metabolic disorders and cancer. AKT2 activity is linked to insulin signalling defects and diabetic complications, while in oncology the kinase has been shown to drive tumour growth and metastasis.
PKBB; PRKBB; HIHGHH; PKBBETA; RAC-BETA
Kinase Activity Assay Usage
For Research Use Only
Kinase Activity Assay Information
Biological Information
The Kinase Enzyme Activity Assay Kit provides a reliable biochemical method for evaluating kinase inhibitors. Built on HitHunter® assay technology, this kit is specifically optimized for AKT2. It comes with all essential components, including recombinant AKT2 (Eurofins #14-339), a substrate, ATP, detection reagents, and assay plates. Each kit is accompanied by a Certificate of Analysis (COA), which outlines the recommended reaction conditions for accurate inhibitor detection. The supplied materials are sufficient to perform experiments covering two, six or twenty five 384-well assay plates.
AKT2 is a serine/threonine kinase and a member of the AKT family, alongside AKT1 and AKT3, collectively referred to as AKT kinase. AKT2 functions as a central node in cellular signalling downstream of PI3K, mediating responses to growth factors such as insulin, IGF1, PDGF, and EGF. It regulates diverse cellular processes including metabolism, proliferation, survival, growth, angiogenesis, cell migration, and differentiation through phosphorylation of multiple downstream substrates.
AKT2 contributes to both metabolic disorders and cancer. AKT2 activity is linked to insulin signalling defects and diabetic complications, while in oncology the kinase has been shown to drive tumour growth and metastasis.
Product Specifications
For Research Use Only
Biological Information
The Kinase Enzyme Activity Assay Kit provides a reliable biochemical method for evaluating kinase inhibitors. Built on HitHunter® assay technology, this kit is specifically optimized for AKT2. It comes with all essential components, including recombinant AKT2 (Eurofins #14-339), a substrate, ATP, detection reagents, and assay plates. Each kit is accompanied by a Certificate of Analysis (COA), which outlines the recommended reaction conditions for accurate inhibitor detection. The supplied materials are sufficient to perform experiments covering two, six or twenty five 384-well assay plates.
AKT2 is a serine/threonine kinase and a member of the AKT family, alongside AKT1 and AKT3, collectively referred to as AKT kinase. AKT2 functions as a central node in cellular signalling downstream of PI3K, mediating responses to growth factors such as insulin, IGF1, PDGF, and EGF. It regulates diverse cellular processes including metabolism, proliferation, survival, growth, angiogenesis, cell migration, and differentiation through phosphorylation of multiple downstream substrates.
AKT2 contributes to both metabolic disorders and cancer. AKT2 activity is linked to insulin signalling defects and diabetic complications, while in oncology the kinase has been shown to drive tumour growth and metastasis.
Product Specifications
For Research Use Only