MSK2 Kinase Enzyme Activity Assay Kit
The MSK2 Kinase Enzyme Activity Assay Kit offers a validated, ready-to-use biochemical assay based on the renowned ADP Hunter™ Plus Assay platform from DiscoverX for accurately measuring kinase inhibitor efficacy. The kit includes recombinant MSK2, substrate, ATP, detection reagents, and assay plates, enabling precise quantification of ADP accumulation. Designed to handle elevated ATP concentrations, this assay delivers robust, reliable data ideal for high-throughput screening (HTS) and other drug discovery applications.
Biological information
The Kinase Enzyme Activity Assay Kit provides a reliable biochemical method for evaluating kinase inhibitors. Built on HitHunter® assay technology, this kit is specifically optimized for MSK2. It comes with all essential components, including recombinant MSK2 (Eurofins #14-616), a substrate, ATP, detection reagents, and assay plates. Each kit is accompanied by a Certificate of Analysis (COA), which outlines the recommended reaction conditions for accurate inhibitor detection. The supplied materials are sufficient to perform experiments covering two, six or twenty five 384-well assay plates.
MSK2 is a mitogen- and stress-activated serine/threonine kinase that works together with MSK1 to couple MAPK signalling to transcriptional and chromatin responses. Mechanistically, MSK2 is activated downstream of ERK1/2 and p38 MAPKs and mediates phosphorylation of transcription factors (e.g. CREB1, ATF1) and chromatin substrates (notably histone H3), thereby regulating stimulus-dependent gene expression. In lipopolysaccharide-stimulated primary macrophages, MSK2 acts downstream of the Toll-like receptor TLR4 to limit the production of pro-inflammatory cytokines.
MSK2 is upregulated in certain cancers, including cervical cancer, and has been shown to promote proliferation and tumour formation.
Product specifications
Chemical data
The Kinase Enzyme Activity Assay Kit provides a reliable biochemical method for evaluating kinase inhibitors. Built on HitHunter® assay technology, this kit is specifically optimized for MSK2. It comes with all essential components, including recombinant MSK2 (Eurofins #14-616), a substrate, ATP, detection reagents, and assay plates. Each kit is accompanied by a Certificate of Analysis (COA), which outlines the recommended reaction conditions for accurate inhibitor detection. The supplied materials are sufficient to perform experiments covering two, six or twenty five 384-well assay plates.
MSK2 is a mitogen- and stress-activated serine/threonine kinase that works together with MSK1 to couple MAPK signalling to transcriptional and chromatin responses. Mechanistically, MSK2 is activated downstream of ERK1/2 and p38 MAPKs and mediates phosphorylation of transcription factors (e.g. CREB1, ATF1) and chromatin substrates (notably histone H3), thereby regulating stimulus-dependent gene expression. In lipopolysaccharide-stimulated primary macrophages, MSK2 acts downstream of the Toll-like receptor TLR4 to limit the production of pro-inflammatory cytokines.
MSK2 is upregulated in certain cancers, including cervical cancer, and has been shown to promote proliferation and tumour formation.
Product specifications
Kinase Activity Assay Biological information
The Kinase Enzyme Activity Assay Kit provides a reliable biochemical method for evaluating kinase inhibitors. Built on HitHunter® assay technology, this kit is specifically optimized for MSK2. It comes with all essential components, including recombinant MSK2 (Eurofins #14-616), a substrate, ATP, detection reagents, and assay plates. Each kit is accompanied by a Certificate of Analysis (COA), which outlines the recommended reaction conditions for accurate inhibitor detection. The supplied materials are sufficient to perform experiments covering two, six or twenty five 384-well assay plates.
MSK2 is a mitogen- and stress-activated serine/threonine kinase that works together with MSK1 to couple MAPK signalling to transcriptional and chromatin responses. Mechanistically, MSK2 is activated downstream of ERK1/2 and p38 MAPKs and mediates phosphorylation of transcription factors (e.g. CREB1, ATF1) and chromatin substrates (notably histone H3), thereby regulating stimulus-dependent gene expression. In lipopolysaccharide-stimulated primary macrophages, MSK2 acts downstream of the Toll-like receptor TLR4 to limit the production of pro-inflammatory cytokines.
MSK2 is upregulated in certain cancers, including cervical cancer, and has been shown to promote proliferation and tumour formation.
MSK2; RSK-B; S6K-alpha-4
Kinase Activity Assay Usage
For Research Use Only
Kinase Activity Assay Information
Biological Information
The Kinase Enzyme Activity Assay Kit provides a reliable biochemical method for evaluating kinase inhibitors. Built on HitHunter® assay technology, this kit is specifically optimized for MSK2. It comes with all essential components, including recombinant MSK2 (Eurofins #14-616), a substrate, ATP, detection reagents, and assay plates. Each kit is accompanied by a Certificate of Analysis (COA), which outlines the recommended reaction conditions for accurate inhibitor detection. The supplied materials are sufficient to perform experiments covering two, six or twenty five 384-well assay plates.
MSK2 is a mitogen- and stress-activated serine/threonine kinase that works together with MSK1 to couple MAPK signalling to transcriptional and chromatin responses. Mechanistically, MSK2 is activated downstream of ERK1/2 and p38 MAPKs and mediates phosphorylation of transcription factors (e.g. CREB1, ATF1) and chromatin substrates (notably histone H3), thereby regulating stimulus-dependent gene expression. In lipopolysaccharide-stimulated primary macrophages, MSK2 acts downstream of the Toll-like receptor TLR4 to limit the production of pro-inflammatory cytokines.
MSK2 is upregulated in certain cancers, including cervical cancer, and has been shown to promote proliferation and tumour formation.
Product Specifications
For Research Use Only
Biological Information
The Kinase Enzyme Activity Assay Kit provides a reliable biochemical method for evaluating kinase inhibitors. Built on HitHunter® assay technology, this kit is specifically optimized for MSK2. It comes with all essential components, including recombinant MSK2 (Eurofins #14-616), a substrate, ATP, detection reagents, and assay plates. Each kit is accompanied by a Certificate of Analysis (COA), which outlines the recommended reaction conditions for accurate inhibitor detection. The supplied materials are sufficient to perform experiments covering two, six or twenty five 384-well assay plates.
MSK2 is a mitogen- and stress-activated serine/threonine kinase that works together with MSK1 to couple MAPK signalling to transcriptional and chromatin responses. Mechanistically, MSK2 is activated downstream of ERK1/2 and p38 MAPKs and mediates phosphorylation of transcription factors (e.g. CREB1, ATF1) and chromatin substrates (notably histone H3), thereby regulating stimulus-dependent gene expression. In lipopolysaccharide-stimulated primary macrophages, MSK2 acts downstream of the Toll-like receptor TLR4 to limit the production of pro-inflammatory cytokines.
MSK2 is upregulated in certain cancers, including cervical cancer, and has been shown to promote proliferation and tumour formation.
Product Specifications
For Research Use Only