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Lck (activated) Kinase Enzyme Activity Assay Kit

The Activated Lck Kinase Enzyme Activity Assay Kit offers a validated, ready-to-use biochemical assay based on the renowned ADP Hunter™ Plus Assay platform from DiscoverX for accurately measuring kinase inhibitor efficacy. The kit includes recombinant activated Lck, substrate, ATP, detection reagents, and assay plates, enabling precise quantification of ADP accumulation. Designed to handle elevated ATP concentrations, this assay delivers robust, reliable data ideal for high-throughput screening (HTS) and other drug discovery applications.

Biological information

Background

The Kinase Enzyme Activity Assay Kit provides a reliable biochemical method for evaluating kinase inhibitors. Built on HitHunter® assay technology, this kit is specifically optimized for Lck. It comes with all essential components, including recombinant Lck (activated) (Eurofins #14-842), a substrate, ATP, detection reagents, and assay plates. Each kit is accompanied by a Certificate of Analysis (COA), which outlines the recommended reaction conditions for accurate inhibitor detection. The supplied materials are sufficient to perform experiments covering two, six or twenty five 384-well assay plates.

Activated lymphocyte-specific protein tyrosine kinase (Lck), also known as p56-LCK, is a Src family kinase required for T-cell receptor signaling and immune cell activation. In its activated state, Lck is a central regulator of T-cell development and immune responses. Dysregulated activity is linked to autoimmunity and hematological cancers. An activated Lck enzyme activity assay kit provides a valuable system for testing inhibitors, advancing research into immune-modulating therapies and cancer immunology.

Target class
Kinase
Family
SRC
Accession number
GenBank NM_005356.3
Target Name
Lck (activated) Kinase Enzyme Activity Assay Kit
Target Alias
LSK; YT16; p56-LCK
Origin
Human
Theori. MW
Affinity tag

Product specifications

Expression system
Purity
Purification method
Sample Buffer
Specified activity
Application
Storage conditions
1 year at -70°C
Usage disclaimer

Chemical data

Background

The Kinase Enzyme Activity Assay Kit provides a reliable biochemical method for evaluating kinase inhibitors. Built on HitHunter® assay technology, this kit is specifically optimized for Lck. It comes with all essential components, including recombinant Lck (activated) (Eurofins #14-842), a substrate, ATP, detection reagents, and assay plates. Each kit is accompanied by a Certificate of Analysis (COA), which outlines the recommended reaction conditions for accurate inhibitor detection. The supplied materials are sufficient to perform experiments covering two, six or twenty five 384-well assay plates.

Activated lymphocyte-specific protein tyrosine kinase (Lck), also known as p56-LCK, is a Src family kinase required for T-cell receptor signaling and immune cell activation. In its activated state, Lck is a central regulator of T-cell development and immune responses. Dysregulated activity is linked to autoimmunity and hematological cancers. An activated Lck enzyme activity assay kit provides a valuable system for testing inhibitors, advancing research into immune-modulating therapies and cancer immunology.

Compound name
Kinase
Catalog number
44-842
Molecular formula
CAS
MW
Ka
Percent composition

Product specifications

Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
Solubility structure

Kinase Activity Assay Biological information

Background

The Kinase Enzyme Activity Assay Kit provides a reliable biochemical method for evaluating kinase inhibitors. Built on HitHunter® assay technology, this kit is specifically optimized for Lck. It comes with all essential components, including recombinant Lck (activated) (Eurofins #14-842), a substrate, ATP, detection reagents, and assay plates. Each kit is accompanied by a Certificate of Analysis (COA), which outlines the recommended reaction conditions for accurate inhibitor detection. The supplied materials are sufficient to perform experiments covering two, six or twenty five 384-well assay plates.

Activated lymphocyte-specific protein tyrosine kinase (Lck), also known as p56-LCK, is a Src family kinase required for T-cell receptor signaling and immune cell activation. In its activated state, Lck is a central regulator of T-cell development and immune responses. Dysregulated activity is linked to autoimmunity and hematological cancers. An activated Lck enzyme activity assay kit provides a valuable system for testing inhibitors, advancing research into immune-modulating therapies and cancer immunology.

Target class
Kinase
Family
SRC
Subfamily
Tyrosine kinase
Protein Name
Lck
Protein Alias
LSK; YT16; p56-LCK
Accession Number
GenBank NM_005356.3
UniProt Number
P06239
Gene Name
LCK
Gene ID
3932
Gene Aliases

LSK; YT16; IMD22; p56lck; pp58lck

Target Species
Human

Kinase Activity Assay Usage

Product Type
Enzyme Activity Assay Kits
Application
Drug Discovery & Development
Storage Conditions
1 year at -70°C
Usage disclaimer

For Research Use Only. These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.

Kinase Activity Assay Information

Assay Type
Enzymatic
Assay Measures
ADP Accumulation

Biological Information

Background

The Kinase Enzyme Activity Assay Kit provides a reliable biochemical method for evaluating kinase inhibitors. Built on HitHunter® assay technology, this kit is specifically optimized for Lck. It comes with all essential components, including recombinant Lck (activated) (Eurofins #14-842), a substrate, ATP, detection reagents, and assay plates. Each kit is accompanied by a Certificate of Analysis (COA), which outlines the recommended reaction conditions for accurate inhibitor detection. The supplied materials are sufficient to perform experiments covering two, six or twenty five 384-well assay plates.

Activated lymphocyte-specific protein tyrosine kinase (Lck), also known as p56-LCK, is a Src family kinase required for T-cell receptor signaling and immune cell activation. In its activated state, Lck is a central regulator of T-cell development and immune responses. Dysregulated activity is linked to autoimmunity and hematological cancers. An activated Lck enzyme activity assay kit provides a valuable system for testing inhibitors, advancing research into immune-modulating therapies and cancer immunology.

Target class
Kinase
Family
SRC
Accession Number
GenBank NM_005356.3
Target Name
Lck
Target Alias
LSK; YT16; p56-LCK
Origin
Human
Theoretical MW
Affinity Tag

Product Specifications

Expression system
Purity
Purification method
Sample Buffer
Enzymatic
Specified activity
Application
Drug Discovery & Development
Storage conditions
1 year at -70°C
Usage disclaimer

For Research Use Only. These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.

Biological Information

Background

The Kinase Enzyme Activity Assay Kit provides a reliable biochemical method for evaluating kinase inhibitors. Built on HitHunter® assay technology, this kit is specifically optimized for Lck. It comes with all essential components, including recombinant Lck (activated) (Eurofins #14-842), a substrate, ATP, detection reagents, and assay plates. Each kit is accompanied by a Certificate of Analysis (COA), which outlines the recommended reaction conditions for accurate inhibitor detection. The supplied materials are sufficient to perform experiments covering two, six or twenty five 384-well assay plates.

Activated lymphocyte-specific protein tyrosine kinase (Lck), also known as p56-LCK, is a Src family kinase required for T-cell receptor signaling and immune cell activation. In its activated state, Lck is a central regulator of T-cell development and immune responses. Dysregulated activity is linked to autoimmunity and hematological cancers. An activated Lck enzyme activity assay kit provides a valuable system for testing inhibitors, advancing research into immune-modulating therapies and cancer immunology.

Target class
Kinase
Family
SRC
Accession Number
GenBank NM_005356.3
Target Name
Lck
Target Alias
LSK; YT16; p56-LCK
Origin
Human
Theoretical MW
Affinity Tag

Product Specifications

Expression system
Purity
Purification method
Sample Buffer
Enzymatic
Specified activity
Application
Drug Discovery & Development
Storage conditions
1 year at -70°C
Usage disclaimer

For Research Use Only. These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.

Datasheets
44-842x Datasheet
User Manual
File N°2
Kinase Enzyme Activity Assay User Manual Rev 1
€ 950,00 EUR
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