HIPK4 Kinase Enzyme Activity Assay Kit
The HIPK4 Kinase Enzyme Activity Assay Kit offers a validated, ready-to-use biochemical assay based on the renowned ADP Hunter™ Plus Assay platform from DiscoverX for accurately measuring kinase inhibitor efficacy. The kit includes recombinant HIPK4, substrate, ATP, detection reagents, and assay plates, enabling precise quantification of ADP accumulation. Designed to handle elevated ATP concentrations, this assay delivers robust, reliable data ideal for high-throughput screening (HTS) and other drug discovery applications.
Biological information
The Kinase Enzyme Activity Assay Kit provides a reliable biochemical method for evaluating kinase inhibitors. Built on HitHunter® assay technology, this kit is specifically optimized for HIPK4. It comes with all essential components, including recombinant HIPK4 (Eurofins #15-003), a substrate, ATP, detection reagents, and assay plates. Each kit is accompanied by a Certificate of Analysis (COA), which outlines the recommended reaction conditions for accurate inhibitor detection. The supplied materials are sufficient to perform experiments covering two, six or twenty five 384-well assay plates.
HIPK4 is a serine/threonine protein kinase in the CMGC family that is essential for male germ cell development and spermiogenesis. Knockout studies have shown that loss of HIPK4 leads to male infertility with defective sperm head shaping and abnormal spermatogenesis. HIPK4 is upregulated in cutaneous squamous cell carcinoma (CSCC) tissues and cell lines where it promotes malignant behaviours such as proliferation, migration and tumour growth by phosphorylating the tumour suppressor TAp63.
Product specifications
Chemical data
The Kinase Enzyme Activity Assay Kit provides a reliable biochemical method for evaluating kinase inhibitors. Built on HitHunter® assay technology, this kit is specifically optimized for HIPK4. It comes with all essential components, including recombinant HIPK4 (Eurofins #15-003), a substrate, ATP, detection reagents, and assay plates. Each kit is accompanied by a Certificate of Analysis (COA), which outlines the recommended reaction conditions for accurate inhibitor detection. The supplied materials are sufficient to perform experiments covering two, six or twenty five 384-well assay plates.
HIPK4 is a serine/threonine protein kinase in the CMGC family that is essential for male germ cell development and spermiogenesis. Knockout studies have shown that loss of HIPK4 leads to male infertility with defective sperm head shaping and abnormal spermatogenesis. HIPK4 is upregulated in cutaneous squamous cell carcinoma (CSCC) tissues and cell lines where it promotes malignant behaviours such as proliferation, migration and tumour growth by phosphorylating the tumour suppressor TAp63.
Product specifications
Kinase Activity Assay Biological information
The Kinase Enzyme Activity Assay Kit provides a reliable biochemical method for evaluating kinase inhibitors. Built on HitHunter® assay technology, this kit is specifically optimized for HIPK4. It comes with all essential components, including recombinant HIPK4 (Eurofins #15-003), a substrate, ATP, detection reagents, and assay plates. Each kit is accompanied by a Certificate of Analysis (COA), which outlines the recommended reaction conditions for accurate inhibitor detection. The supplied materials are sufficient to perform experiments covering two, six or twenty five 384-well assay plates.
HIPK4 is a serine/threonine protein kinase in the CMGC family that is essential for male germ cell development and spermiogenesis. Knockout studies have shown that loss of HIPK4 leads to male infertility with defective sperm head shaping and abnormal spermatogenesis. HIPK4 is upregulated in cutaneous squamous cell carcinoma (CSCC) tissues and cell lines where it promotes malignant behaviours such as proliferation, migration and tumour growth by phosphorylating the tumour suppressor TAp63.
Kinase Activity Assay Usage
For Research Use Only
Kinase Activity Assay Information
Biological Information
The Kinase Enzyme Activity Assay Kit provides a reliable biochemical method for evaluating kinase inhibitors. Built on HitHunter® assay technology, this kit is specifically optimized for HIPK4. It comes with all essential components, including recombinant HIPK4 (Eurofins #15-003), a substrate, ATP, detection reagents, and assay plates. Each kit is accompanied by a Certificate of Analysis (COA), which outlines the recommended reaction conditions for accurate inhibitor detection. The supplied materials are sufficient to perform experiments covering two, six or twenty five 384-well assay plates.
HIPK4 is a serine/threonine protein kinase in the CMGC family that is essential for male germ cell development and spermiogenesis. Knockout studies have shown that loss of HIPK4 leads to male infertility with defective sperm head shaping and abnormal spermatogenesis. HIPK4 is upregulated in cutaneous squamous cell carcinoma (CSCC) tissues and cell lines where it promotes malignant behaviours such as proliferation, migration and tumour growth by phosphorylating the tumour suppressor TAp63.
Product Specifications
For Research Use Only
Biological Information
The Kinase Enzyme Activity Assay Kit provides a reliable biochemical method for evaluating kinase inhibitors. Built on HitHunter® assay technology, this kit is specifically optimized for HIPK4. It comes with all essential components, including recombinant HIPK4 (Eurofins #15-003), a substrate, ATP, detection reagents, and assay plates. Each kit is accompanied by a Certificate of Analysis (COA), which outlines the recommended reaction conditions for accurate inhibitor detection. The supplied materials are sufficient to perform experiments covering two, six or twenty five 384-well assay plates.
HIPK4 is a serine/threonine protein kinase in the CMGC family that is essential for male germ cell development and spermiogenesis. Knockout studies have shown that loss of HIPK4 leads to male infertility with defective sperm head shaping and abnormal spermatogenesis. HIPK4 is upregulated in cutaneous squamous cell carcinoma (CSCC) tissues and cell lines where it promotes malignant behaviours such as proliferation, migration and tumour growth by phosphorylating the tumour suppressor TAp63.
Product Specifications
For Research Use Only