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CK1 delta Kinase Enzyme Activity Assay Kit

The CK1 delta Kinase Enzyme Activity Assay Kit offers a validated, ready-to-use biochemical assay based on the renowned ADP Hunter™ Plus Assay platform from DiscoverX for accurately measuring kinase inhibitor efficacy. The kit includes recombinant CK1 delta, substrate, ATP, detection reagents, and assay plates, enabling precise quantification of ADP accumulation. Designed to handle elevated ATP concentrations, this assay delivers robust, reliable data ideal for high-throughput screening (HTS) and other drug discovery applications.

Biological information

Background

The Kinase Enzyme Activity Assay Kit provides a reliable biochemical method for evaluating kinase inhibitors. Built on HitHunter® assay technology, this kit is specifically optimized for CK1delta. It comes with all essential components, including recombinant CK1delta (Eurofins #14-520), a substrate, ATP, detection reagents, and assay plates. Each kit is accompanied by a Certificate of Analysis (COA), which outlines the recommended reaction conditions for accurate inhibitor detection. The supplied materials are sufficient to perform experiments covering two, six or twenty five 384-well assay plates.

Casein kinase I delta (CK1δ), also referred to as CSNK1D, is a serine/threonine kinase that regulates circadian rhythm, Wnt signaling, and cell division. Dysregulated CK1δ function has been linked to cancer, Alzheimer’s disease, and sleep disorders, making it an emerging target in drug discovery and neurological research. A CK1 delta enzyme activity assay kit allows for accurate assessment of enzyme function and inhibitor effects, facilitating the development of therapies targeting CK1δ-regulated signaling pathways.

Target class
Kinase
Family
CK1
Accession number
GenBank U29171.1
Target Name
CK1 delta Kinase Enzyme Activity Assay Kit
Target Alias
CSNK1D
Origin
Human
Theori. MW
Affinity tag

Product specifications

Expression system
Purity
Purification method
Sample Buffer
Specified activity
Application
Storage conditions
1 year at -70°C
Usage disclaimer

Chemical data

Background

The Kinase Enzyme Activity Assay Kit provides a reliable biochemical method for evaluating kinase inhibitors. Built on HitHunter® assay technology, this kit is specifically optimized for CK1delta. It comes with all essential components, including recombinant CK1delta (Eurofins #14-520), a substrate, ATP, detection reagents, and assay plates. Each kit is accompanied by a Certificate of Analysis (COA), which outlines the recommended reaction conditions for accurate inhibitor detection. The supplied materials are sufficient to perform experiments covering two, six or twenty five 384-well assay plates.

Casein kinase I delta (CK1δ), also referred to as CSNK1D, is a serine/threonine kinase that regulates circadian rhythm, Wnt signaling, and cell division. Dysregulated CK1δ function has been linked to cancer, Alzheimer’s disease, and sleep disorders, making it an emerging target in drug discovery and neurological research. A CK1 delta enzyme activity assay kit allows for accurate assessment of enzyme function and inhibitor effects, facilitating the development of therapies targeting CK1δ-regulated signaling pathways.

Compound name
Kinase
Catalog number
44-520
Molecular formula
CAS
MW
Ka
Percent composition

Product specifications

Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
Solubility structure

Kinase Activity Assay Biological information

Background

The Kinase Enzyme Activity Assay Kit provides a reliable biochemical method for evaluating kinase inhibitors. Built on HitHunter® assay technology, this kit is specifically optimized for CK1delta. It comes with all essential components, including recombinant CK1delta (Eurofins #14-520), a substrate, ATP, detection reagents, and assay plates. Each kit is accompanied by a Certificate of Analysis (COA), which outlines the recommended reaction conditions for accurate inhibitor detection. The supplied materials are sufficient to perform experiments covering two, six or twenty five 384-well assay plates.

Casein kinase I delta (CK1δ), also referred to as CSNK1D, is a serine/threonine kinase that regulates circadian rhythm, Wnt signaling, and cell division. Dysregulated CK1δ function has been linked to cancer, Alzheimer’s disease, and sleep disorders, making it an emerging target in drug discovery and neurological research. A CK1 delta enzyme activity assay kit allows for accurate assessment of enzyme function and inhibitor effects, facilitating the development of therapies targeting CK1δ-regulated signaling pathways.

Target class
Kinase
Family
CK1
Subfamily
Serine/threonine kinase
Protein Name
CK1d
Protein Alias
CSNK1D
Accession Number
GenBank U29171.1
UniProt Number
P48730
Gene Name
CSNK1D
Gene ID
1453
Gene Aliases

ASPS; CKId; HCKID; FASPS2; CKIdelta; CKI-delta

Target Species
Human

Kinase Activity Assay Usage

Product Type
Enzyme Activity Assay Kits
Application
Drug Discovery & Development
Storage Conditions
1 year at -70°C
Usage disclaimer

For Research Use Only. These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.

Kinase Activity Assay Information

Assay Type
Enzymatic
Assay Measures
ADP Accumulation

Biological Information

Background

The Kinase Enzyme Activity Assay Kit provides a reliable biochemical method for evaluating kinase inhibitors. Built on HitHunter® assay technology, this kit is specifically optimized for CK1delta. It comes with all essential components, including recombinant CK1delta (Eurofins #14-520), a substrate, ATP, detection reagents, and assay plates. Each kit is accompanied by a Certificate of Analysis (COA), which outlines the recommended reaction conditions for accurate inhibitor detection. The supplied materials are sufficient to perform experiments covering two, six or twenty five 384-well assay plates.

Casein kinase I delta (CK1δ), also referred to as CSNK1D, is a serine/threonine kinase that regulates circadian rhythm, Wnt signaling, and cell division. Dysregulated CK1δ function has been linked to cancer, Alzheimer’s disease, and sleep disorders, making it an emerging target in drug discovery and neurological research. A CK1 delta enzyme activity assay kit allows for accurate assessment of enzyme function and inhibitor effects, facilitating the development of therapies targeting CK1δ-regulated signaling pathways.

Target class
Kinase
Family
CK1
Accession Number
GenBank U29171.1
Target Name
CK1d
Target Alias
CSNK1D
Origin
Human
Theoretical MW
Affinity Tag

Product Specifications

Expression system
Purity
Purification method
Sample Buffer
Enzymatic
Specified activity
Application
Drug Discovery & Development
Storage conditions
1 year at -70°C
Usage disclaimer

For Research Use Only. These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.

Biological Information

Background

The Kinase Enzyme Activity Assay Kit provides a reliable biochemical method for evaluating kinase inhibitors. Built on HitHunter® assay technology, this kit is specifically optimized for CK1delta. It comes with all essential components, including recombinant CK1delta (Eurofins #14-520), a substrate, ATP, detection reagents, and assay plates. Each kit is accompanied by a Certificate of Analysis (COA), which outlines the recommended reaction conditions for accurate inhibitor detection. The supplied materials are sufficient to perform experiments covering two, six or twenty five 384-well assay plates.

Casein kinase I delta (CK1δ), also referred to as CSNK1D, is a serine/threonine kinase that regulates circadian rhythm, Wnt signaling, and cell division. Dysregulated CK1δ function has been linked to cancer, Alzheimer’s disease, and sleep disorders, making it an emerging target in drug discovery and neurological research. A CK1 delta enzyme activity assay kit allows for accurate assessment of enzyme function and inhibitor effects, facilitating the development of therapies targeting CK1δ-regulated signaling pathways.

Target class
Kinase
Family
CK1
Accession Number
GenBank U29171.1
Target Name
CK1d
Target Alias
CSNK1D
Origin
Human
Theoretical MW
Affinity Tag

Product Specifications

Expression system
Purity
Purification method
Sample Buffer
Enzymatic
Specified activity
Application
Drug Discovery & Development
Storage conditions
1 year at -70°C
Usage disclaimer

For Research Use Only. These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.

Datasheets
44-520x Datasheet
User Manual
File N°2
Kinase Enzyme Activity Assay User Manual Rev 1
€ 950,00 EUR
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