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CDKL5 Kinase Enzyme Activity Assay Kit

The CDKL5 Kinase Enzyme Activity Assay Kit offers a validated, ready-to-use biochemical assay based on the renowned ADP Hunter™ Plus Assay platform from DiscoverX for accurately measuring kinase inhibitor efficacy. The kit includes recombinant CDKL5, substrate, ATP, detection reagents, and assay plates, enabling precise quantification of ADP accumulation. Designed to handle elevated ATP concentrations, this assay delivers robust, reliable data ideal for high-throughput screening (HTS) and other drug discovery applications.

Biological information

Background

The Kinase Enzyme Activity Assay Kit provides a reliable biochemical method for evaluating kinase inhibitors. Built on HitHunter® assay technology, this kit is specifically optimized for CDKL5. It comes with all essential components, including recombinant CDKL5 (Eurofins #14-998), a substrate, ATP, detection reagents, and assay plates. Each kit is accompanied by a Certificate of Analysis (COA), which outlines the recommended reaction conditions for accurate inhibitor detection. The supplied materials are sufficient to perform experiments covering two, six or twenty five 384-well assay plates.

Cyclin-dependent kinase-like 5 (CDKL5) is a serine/threonine kinase expressed mainly in the brain, where it regulates neuronal signaling and synaptic development. Mutations in CDKL5 cause CDKL5 deficiency disorder, a severe form of epileptic encephalopathy. A CDKL5 enzyme activity assay kit provides researchers with a valuable system to study kinase activity and inhibitors, accelerating the search for treatments for rare neurological diseases.

Target class
Kinase
Family
CMGC
Accession number
GenBank NM_003159.3
Target Name
CDKL5 Kinase Enzyme Activity Assay Kit
Target Alias
STK9
Origin
Human
Theori. MW
Affinity tag

Product specifications

Expression system
Purity
Purification method
Sample Buffer
Specified activity
Application
Storage conditions
1 year at -70°C
Usage disclaimer

Chemical data

Background

The Kinase Enzyme Activity Assay Kit provides a reliable biochemical method for evaluating kinase inhibitors. Built on HitHunter® assay technology, this kit is specifically optimized for CDKL5. It comes with all essential components, including recombinant CDKL5 (Eurofins #14-998), a substrate, ATP, detection reagents, and assay plates. Each kit is accompanied by a Certificate of Analysis (COA), which outlines the recommended reaction conditions for accurate inhibitor detection. The supplied materials are sufficient to perform experiments covering two, six or twenty five 384-well assay plates.

Cyclin-dependent kinase-like 5 (CDKL5) is a serine/threonine kinase expressed mainly in the brain, where it regulates neuronal signaling and synaptic development. Mutations in CDKL5 cause CDKL5 deficiency disorder, a severe form of epileptic encephalopathy. A CDKL5 enzyme activity assay kit provides researchers with a valuable system to study kinase activity and inhibitors, accelerating the search for treatments for rare neurological diseases.

Compound name
Kinase
Catalog number
44-998
Molecular formula
CAS
MW
Ka
Percent composition

Product specifications

Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
Solubility structure

Kinase Activity Assay Biological information

Background

The Kinase Enzyme Activity Assay Kit provides a reliable biochemical method for evaluating kinase inhibitors. Built on HitHunter® assay technology, this kit is specifically optimized for CDKL5. It comes with all essential components, including recombinant CDKL5 (Eurofins #14-998), a substrate, ATP, detection reagents, and assay plates. Each kit is accompanied by a Certificate of Analysis (COA), which outlines the recommended reaction conditions for accurate inhibitor detection. The supplied materials are sufficient to perform experiments covering two, six or twenty five 384-well assay plates.

Cyclin-dependent kinase-like 5 (CDKL5) is a serine/threonine kinase expressed mainly in the brain, where it regulates neuronal signaling and synaptic development. Mutations in CDKL5 cause CDKL5 deficiency disorder, a severe form of epileptic encephalopathy. A CDKL5 enzyme activity assay kit provides researchers with a valuable system to study kinase activity and inhibitors, accelerating the search for treatments for rare neurological diseases.

Target class
Kinase
Family
CMGC
Subfamily
Serine/threonine kinase
Protein Name
CDKL5
Protein Alias
STK9
Accession Number
GenBank NM_003159.3
UniProt Number
O76039
Gene Name
CDKL5
Gene ID
6792
Gene Aliases

DEE2; ISSX; STK9; EIEE2; CFAP247

Target Species
Human

Kinase Activity Assay Usage

Product Type
Enzyme Activity Assay Kits
Application
Drug Discovery & Development
Storage Conditions
1 year at -70°C
Usage disclaimer

For Research Use Only. These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.

Kinase Activity Assay Information

Assay Type
Assay Measures
ADP Accumulation

Biological Information

Background

The Kinase Enzyme Activity Assay Kit provides a reliable biochemical method for evaluating kinase inhibitors. Built on HitHunter® assay technology, this kit is specifically optimized for CDKL5. It comes with all essential components, including recombinant CDKL5 (Eurofins #14-998), a substrate, ATP, detection reagents, and assay plates. Each kit is accompanied by a Certificate of Analysis (COA), which outlines the recommended reaction conditions for accurate inhibitor detection. The supplied materials are sufficient to perform experiments covering two, six or twenty five 384-well assay plates.

Cyclin-dependent kinase-like 5 (CDKL5) is a serine/threonine kinase expressed mainly in the brain, where it regulates neuronal signaling and synaptic development. Mutations in CDKL5 cause CDKL5 deficiency disorder, a severe form of epileptic encephalopathy. A CDKL5 enzyme activity assay kit provides researchers with a valuable system to study kinase activity and inhibitors, accelerating the search for treatments for rare neurological diseases.

Target class
Kinase
Family
CMGC
Accession Number
GenBank NM_003159.3
Target Name
CDKL5
Target Alias
STK9
Origin
Human
Theoretical MW
Affinity Tag

Product Specifications

Expression system
Purity
Purification method
Sample Buffer
Specified activity
Application
Drug Discovery & Development
Storage conditions
1 year at -70°C
Usage disclaimer

For Research Use Only. These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.

Biological Information

Background

The Kinase Enzyme Activity Assay Kit provides a reliable biochemical method for evaluating kinase inhibitors. Built on HitHunter® assay technology, this kit is specifically optimized for CDKL5. It comes with all essential components, including recombinant CDKL5 (Eurofins #14-998), a substrate, ATP, detection reagents, and assay plates. Each kit is accompanied by a Certificate of Analysis (COA), which outlines the recommended reaction conditions for accurate inhibitor detection. The supplied materials are sufficient to perform experiments covering two, six or twenty five 384-well assay plates.

Cyclin-dependent kinase-like 5 (CDKL5) is a serine/threonine kinase expressed mainly in the brain, where it regulates neuronal signaling and synaptic development. Mutations in CDKL5 cause CDKL5 deficiency disorder, a severe form of epileptic encephalopathy. A CDKL5 enzyme activity assay kit provides researchers with a valuable system to study kinase activity and inhibitors, accelerating the search for treatments for rare neurological diseases.

Target class
Kinase
Family
CMGC
Accession Number
GenBank NM_003159.3
Target Name
CDKL5
Target Alias
STK9
Origin
Human
Theoretical MW
Affinity Tag

Product Specifications

Expression system
Purity
Purification method
Sample Buffer
Specified activity
Application
Drug Discovery & Development
Storage conditions
1 year at -70°C
Usage disclaimer

For Research Use Only. These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.

Datasheets
44-998x Datasheet
User Manual
File N°2
Kinase Enzyme Activity Assay User Manual Rev 1
€ 950,00 EUR
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