Brought to you by:

BTK Kinase Enzyme Activity Assay Kit

The BTK Kinase Enzyme Activity Assay Kit offers a validated, ready-to-use biochemical assay based on the renowned ADP Hunter™ Plus Assay platform from DiscoverX for accurately measuring kinase inhibitor efficacy. The kit includes recombinant BTK, substrate, ATP, detection reagents, and assay plates, enabling precise quantification of ADP accumulation. Designed to handle elevated ATP concentrations, this assay delivers robust, reliable data ideal for high-throughput screening (HTS) and other drug discovery applications.

Biological information

Background

The Kinase Enzyme Activity Assay Kit provides a reliable biochemical method for evaluating kinase inhibitors. Built on HitHunter® assay technology, this kit is specifically optimized for BTK. It comes with all essential components, including recombinant BTK (Eurofins #14-552), a substrate, ATP, detection reagents, and assay plates. Each kit is accompanied by a Certificate of Analysis (COA), which outlines the recommended reaction conditions for accurate inhibitor detection. The supplied materials are sufficient to perform experiments covering two, six or twenty five 384-well assay plates.

Bruton’s tyrosine kinase (BTK) is a non-receptor tyrosine kinase from the Tec family, essential for B-cell receptor signaling and B-cell development. Mutations in BTK cause X-linked agammaglobulinemia (XLA), while overactivation is implicated in B-cell malignancies and autoimmune diseases. A BTK enzyme activity assay kit enables precise measurement of kinase activity and inhibitor screening, supporting the development of BTK-targeted therapies for leukemia, lymphoma, and inflammatory disorders.

Target class
Kinase
Family
TEC
Accession number
GenBank NM_000061.1
Target Name
BTK Kinase Enzyme Activity Assay Kit
Target Alias
ATK; BPK
Origin
Human
Theori. MW
Affinity tag

Product specifications

Expression system
Purity
Purification method
Sample Buffer
Specified activity
Application
Storage conditions
1 year at -70°C
Usage disclaimer

Chemical data

Background

The Kinase Enzyme Activity Assay Kit provides a reliable biochemical method for evaluating kinase inhibitors. Built on HitHunter® assay technology, this kit is specifically optimized for BTK. It comes with all essential components, including recombinant BTK (Eurofins #14-552), a substrate, ATP, detection reagents, and assay plates. Each kit is accompanied by a Certificate of Analysis (COA), which outlines the recommended reaction conditions for accurate inhibitor detection. The supplied materials are sufficient to perform experiments covering two, six or twenty five 384-well assay plates.

Bruton’s tyrosine kinase (BTK) is a non-receptor tyrosine kinase from the Tec family, essential for B-cell receptor signaling and B-cell development. Mutations in BTK cause X-linked agammaglobulinemia (XLA), while overactivation is implicated in B-cell malignancies and autoimmune diseases. A BTK enzyme activity assay kit enables precise measurement of kinase activity and inhibitor screening, supporting the development of BTK-targeted therapies for leukemia, lymphoma, and inflammatory disorders.

Compound name
Kinase
Catalog number
44-552
Molecular formula
CAS
MW
Ka
Percent composition

Product specifications

Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
Solubility structure

Kinase Activity Assay Biological information

Background

The Kinase Enzyme Activity Assay Kit provides a reliable biochemical method for evaluating kinase inhibitors. Built on HitHunter® assay technology, this kit is specifically optimized for BTK. It comes with all essential components, including recombinant BTK (Eurofins #14-552), a substrate, ATP, detection reagents, and assay plates. Each kit is accompanied by a Certificate of Analysis (COA), which outlines the recommended reaction conditions for accurate inhibitor detection. The supplied materials are sufficient to perform experiments covering two, six or twenty five 384-well assay plates.

Bruton’s tyrosine kinase (BTK) is a non-receptor tyrosine kinase from the Tec family, essential for B-cell receptor signaling and B-cell development. Mutations in BTK cause X-linked agammaglobulinemia (XLA), while overactivation is implicated in B-cell malignancies and autoimmune diseases. A BTK enzyme activity assay kit enables precise measurement of kinase activity and inhibitor screening, supporting the development of BTK-targeted therapies for leukemia, lymphoma, and inflammatory disorders.

Target class
Kinase
Family
TEC
Subfamily
Tyrosine kinase
Protein Name
BTK
Protein Alias
ATK; BPK
Accession Number
GenBank NM_000061.1
UniProt Number
Q06187
Gene Name
BTK
Gene ID
695
Gene Aliases

AT; ATK; BPK; XLA; IMD1; AGMX1; IGHD3; PSCTK1

Target Species
Human

Kinase Activity Assay Usage

Product Type
Enzyme Activity Assay Kits
Application
Drug Discovery & Development
Storage Conditions
1 year at -70°C
Usage disclaimer

For Research Use Only. These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.

Kinase Activity Assay Information

Assay Type
ADP Accumulation
Assay Measures

Biological Information

Background

The Kinase Enzyme Activity Assay Kit provides a reliable biochemical method for evaluating kinase inhibitors. Built on HitHunter® assay technology, this kit is specifically optimized for BTK. It comes with all essential components, including recombinant BTK (Eurofins #14-552), a substrate, ATP, detection reagents, and assay plates. Each kit is accompanied by a Certificate of Analysis (COA), which outlines the recommended reaction conditions for accurate inhibitor detection. The supplied materials are sufficient to perform experiments covering two, six or twenty five 384-well assay plates.

Bruton’s tyrosine kinase (BTK) is a non-receptor tyrosine kinase from the Tec family, essential for B-cell receptor signaling and B-cell development. Mutations in BTK cause X-linked agammaglobulinemia (XLA), while overactivation is implicated in B-cell malignancies and autoimmune diseases. A BTK enzyme activity assay kit enables precise measurement of kinase activity and inhibitor screening, supporting the development of BTK-targeted therapies for leukemia, lymphoma, and inflammatory disorders.

Target class
Kinase
Family
TEC
Accession Number
GenBank NM_000061.1
Target Name
BTK
Target Alias
ATK; BPK
Origin
Human
Theoretical MW
Affinity Tag

Product Specifications

Expression system
Purity
Purification method
Sample Buffer
ADP Accumulation
Specified activity
Application
Drug Discovery & Development
Storage conditions
1 year at -70°C
Usage disclaimer

For Research Use Only. These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.

Biological Information

Background

The Kinase Enzyme Activity Assay Kit provides a reliable biochemical method for evaluating kinase inhibitors. Built on HitHunter® assay technology, this kit is specifically optimized for BTK. It comes with all essential components, including recombinant BTK (Eurofins #14-552), a substrate, ATP, detection reagents, and assay plates. Each kit is accompanied by a Certificate of Analysis (COA), which outlines the recommended reaction conditions for accurate inhibitor detection. The supplied materials are sufficient to perform experiments covering two, six or twenty five 384-well assay plates.

Bruton’s tyrosine kinase (BTK) is a non-receptor tyrosine kinase from the Tec family, essential for B-cell receptor signaling and B-cell development. Mutations in BTK cause X-linked agammaglobulinemia (XLA), while overactivation is implicated in B-cell malignancies and autoimmune diseases. A BTK enzyme activity assay kit enables precise measurement of kinase activity and inhibitor screening, supporting the development of BTK-targeted therapies for leukemia, lymphoma, and inflammatory disorders.

Target class
Kinase
Family
TEC
Accession Number
GenBank NM_000061.1
Target Name
BTK
Target Alias
ATK; BPK
Origin
Human
Theoretical MW
Affinity Tag

Product Specifications

Expression system
Purity
Purification method
Sample Buffer
ADP Accumulation
Specified activity
Application
Drug Discovery & Development
Storage conditions
1 year at -70°C
Usage disclaimer

For Research Use Only. These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.

Datasheets
44-552x Datasheet
User Manual
File N°2
Kinase Enzyme Activity Assay User Manual Rev 1
€ 950,00 EUR
Purchase from our distributor
Button