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AMPK (alpha1, beta1, gamma1) Kinase Enzyme Activity Assay Kit

The AMPK (alpha1, beta1, gamma1) Kinase Enzyme Activity Assay Kit offers a validated, ready-to-use biochemical assay based on the renowned ADP Hunter™ Plus Assay platform from DiscoverX for accurately measuring kinase inhibitor efficacy. The kit includes recombinant AMPK complex, substrate, ATP, detection reagents, and assay plates, enabling precise quantification of ADP accumulation. Designed to handle elevated ATP concentrations, this assay delivers robust, reliable data ideal for high-throughput screening (HTS) and other drug discovery applications.

Biological information

Background

The Kinase Enzyme Activity Assay Kit provides a reliable biochemical method for evaluating kinase inhibitors. Built on HitHunter® assay technology, this kit is specifically optimized for AMPK (α1, β1, γ1). It comes with all essential components, including recombinant AMPK (α1, β1, γ1) (Eurofins #14-840), a substrate, ATP, detection reagents, and assay plates. Each kit is accompanied by a Certificate of Analysis (COA), which outlines the recommended reaction conditions for accurate inhibitor detection. The supplied materials are sufficient to perform experiments covering two, six or twenty five 384-well assay plates.

AMP-activated protein kinase (AMPK) is a heterotrimeric serine/threonine kinase complex composed of α, β, and γ subunits. AMPK is a master regulator of cellular energy homeostasis, activated in response to metabolic stress. Dysregulated AMPK signaling is implicated in diabetes, obesity, cardiovascular disease, and cancer. An AMPK enzyme activity assay kit enables precise analysis of this critical metabolic sensor, supporting drug discovery for metabolic disorders and oncology.

Target class
Kinase
Family
CAMK
Accession number
GenBank AF100763.1 (AMPKalpha1); GenBank NM_006253.2 (AMPKbeta1); GenBank NM_002733 (AMPKgamma1)
Target Name
AMPK (alpha1, beta1, gamma1) Kinase Enzyme Activity Assay Kit
Target Alias
PRKAA1
Origin
Human
Theori. MW
Affinity tag

Product specifications

Expression system
Purity
Purification method
Sample Buffer
Specified activity
Application
Storage conditions
1 year at -70°C
Usage disclaimer

Chemical data

Background

The Kinase Enzyme Activity Assay Kit provides a reliable biochemical method for evaluating kinase inhibitors. Built on HitHunter® assay technology, this kit is specifically optimized for AMPK (α1, β1, γ1). It comes with all essential components, including recombinant AMPK (α1, β1, γ1) (Eurofins #14-840), a substrate, ATP, detection reagents, and assay plates. Each kit is accompanied by a Certificate of Analysis (COA), which outlines the recommended reaction conditions for accurate inhibitor detection. The supplied materials are sufficient to perform experiments covering two, six or twenty five 384-well assay plates.

AMP-activated protein kinase (AMPK) is a heterotrimeric serine/threonine kinase complex composed of α, β, and γ subunits. AMPK is a master regulator of cellular energy homeostasis, activated in response to metabolic stress. Dysregulated AMPK signaling is implicated in diabetes, obesity, cardiovascular disease, and cancer. An AMPK enzyme activity assay kit enables precise analysis of this critical metabolic sensor, supporting drug discovery for metabolic disorders and oncology.

Compound name
Kinase
Catalog number
44-840
Molecular formula
CAS
MW
Ka
Percent composition

Product specifications

Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
Solubility structure

Kinase Activity Assay Biological information

Background

The Kinase Enzyme Activity Assay Kit provides a reliable biochemical method for evaluating kinase inhibitors. Built on HitHunter® assay technology, this kit is specifically optimized for AMPK (α1, β1, γ1). It comes with all essential components, including recombinant AMPK (α1, β1, γ1) (Eurofins #14-840), a substrate, ATP, detection reagents, and assay plates. Each kit is accompanied by a Certificate of Analysis (COA), which outlines the recommended reaction conditions for accurate inhibitor detection. The supplied materials are sufficient to perform experiments covering two, six or twenty five 384-well assay plates.

AMP-activated protein kinase (AMPK) is a heterotrimeric serine/threonine kinase complex composed of α, β, and γ subunits. AMPK is a master regulator of cellular energy homeostasis, activated in response to metabolic stress. Dysregulated AMPK signaling is implicated in diabetes, obesity, cardiovascular disease, and cancer. An AMPK enzyme activity assay kit enables precise analysis of this critical metabolic sensor, supporting drug discovery for metabolic disorders and oncology.

Target class
Kinase
Family
CAMK
Subfamily
Serine/threonine kinase
Protein Name
AMPK
Protein Alias
PRKAA1
Accession Number
GenBank AF100763.1 (AMPKalpha1); GenBank NM_006253.2 (AMPKbeta1); GenBank NM_002733 (AMPKgamma1)
UniProt Number
Q13131
Gene Name
PRKAA1
Gene ID
5562
Gene Aliases

AMPK; AMPKa1; AMPK alpha 1

Target Species
Human

Kinase Activity Assay Usage

Product Type
Enzyme Activity Assay Kits
Application
Drug Discovery & Development
Storage Conditions
1 year at -70°C
Usage disclaimer

For Research Use Only. These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.

Kinase Activity Assay Information

Assay Type
Assay Measures
ADP Accumulation

Biological Information

Background

The Kinase Enzyme Activity Assay Kit provides a reliable biochemical method for evaluating kinase inhibitors. Built on HitHunter® assay technology, this kit is specifically optimized for AMPK (α1, β1, γ1). It comes with all essential components, including recombinant AMPK (α1, β1, γ1) (Eurofins #14-840), a substrate, ATP, detection reagents, and assay plates. Each kit is accompanied by a Certificate of Analysis (COA), which outlines the recommended reaction conditions for accurate inhibitor detection. The supplied materials are sufficient to perform experiments covering two, six or twenty five 384-well assay plates.

AMP-activated protein kinase (AMPK) is a heterotrimeric serine/threonine kinase complex composed of α, β, and γ subunits. AMPK is a master regulator of cellular energy homeostasis, activated in response to metabolic stress. Dysregulated AMPK signaling is implicated in diabetes, obesity, cardiovascular disease, and cancer. An AMPK enzyme activity assay kit enables precise analysis of this critical metabolic sensor, supporting drug discovery for metabolic disorders and oncology.

Target class
Kinase
Family
CAMK
Accession Number
GenBank AF100763.1 (AMPKalpha1); GenBank NM_006253.2 (AMPKbeta1); GenBank NM_002733 (AMPKgamma1)
Target Name
AMPK
Target Alias
PRKAA1
Origin
Human
Theoretical MW
Affinity Tag

Product Specifications

Expression system
Purity
Purification method
Sample Buffer
Specified activity
Application
Drug Discovery & Development
Storage conditions
1 year at -70°C
Usage disclaimer

For Research Use Only. These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.

Biological Information

Background

The Kinase Enzyme Activity Assay Kit provides a reliable biochemical method for evaluating kinase inhibitors. Built on HitHunter® assay technology, this kit is specifically optimized for AMPK (α1, β1, γ1). It comes with all essential components, including recombinant AMPK (α1, β1, γ1) (Eurofins #14-840), a substrate, ATP, detection reagents, and assay plates. Each kit is accompanied by a Certificate of Analysis (COA), which outlines the recommended reaction conditions for accurate inhibitor detection. The supplied materials are sufficient to perform experiments covering two, six or twenty five 384-well assay plates.

AMP-activated protein kinase (AMPK) is a heterotrimeric serine/threonine kinase complex composed of α, β, and γ subunits. AMPK is a master regulator of cellular energy homeostasis, activated in response to metabolic stress. Dysregulated AMPK signaling is implicated in diabetes, obesity, cardiovascular disease, and cancer. An AMPK enzyme activity assay kit enables precise analysis of this critical metabolic sensor, supporting drug discovery for metabolic disorders and oncology.

Target class
Kinase
Family
CAMK
Accession Number
GenBank AF100763.1 (AMPKalpha1); GenBank NM_006253.2 (AMPKbeta1); GenBank NM_002733 (AMPKgamma1)
Target Name
AMPK
Target Alias
PRKAA1
Origin
Human
Theoretical MW
Affinity Tag

Product Specifications

Expression system
Purity
Purification method
Sample Buffer
Specified activity
Application
Drug Discovery & Development
Storage conditions
1 year at -70°C
Usage disclaimer

For Research Use Only. These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.

Datasheets
44-840x Datasheet
User Manual
File N°2
Kinase Enzyme Activity Assay User Manual Rev 1
€ 1 300,00 EUR
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