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AKT1/PKB alpha Kinase Enzyme Activity Assay Kit

The AKT1/PKB alpha Kinase Enzyme Activity Assay Kit offers a validated, ready-to-use biochemical assay based on the renowned ADP Hunter™ Plus Assay platform from DiscoverX for accurately measuring kinase inhibitor efficacy. The kit includes recombinant AKT1/PKB alpha, substrate, ATP, detection reagents, and assay plates, enabling precise quantification of ADP accumulation. Designed to handle elevated ATP concentrations, this assay delivers robust, reliable data ideal for high-throughput screening (HTS) and other drug discovery applications.

Biological information

Background

The Kinase Enzyme Activity Assay Kit provides a reliable biochemical method for evaluating kinase inhibitors. Built on HitHunter® assay technology, this kit is specifically optimized for Akt1/PKBalpha. It comes with all essential components, including recombinant Akt1/PKBalpha (Eurofins #14-276), a substrate, ATP, detection reagents, and assay plates. Each kit is accompanied by a Certificate of Analysis (COA), which outlines the recommended reaction conditions for accurate inhibitor detection. The supplied materials are sufficient to perform experiments covering two, six or twenty five 384-well assay plates.

Akt1, also known as protein kinase B alpha (PKBα), is a serine/threonine kinase that plays a central role in cell growth, proliferation, survival, and metabolism. Dysregulation of Akt1 signaling has been linked to multiple diseases, including cancer, diabetes, and cardiovascular disorders, making it a critical target in drug discovery and kinase inhibitor research. The availability of a ready-to-use Akt1 enzyme activity assay kit allows researchers to study this pathway with precision, accelerating the development of novel therapeutics targeting the PI3K/Akt signaling pathway.

Target class
Kinase
Family
AGC
Accession number
GenBank M63167.1
Target Name
AKT1/PKB alpha Kinase Enzyme Activity Assay Kit
Target Alias
PKBalpha; RAC-PK-alpha; c-Akt
Origin
Human
Theori. MW
Affinity tag

Product specifications

Expression system
Purity
Purification method
Sample Buffer
Specified activity
Application
Storage conditions
1 year at -70°C
Usage disclaimer

Chemical data

Background

The Kinase Enzyme Activity Assay Kit provides a reliable biochemical method for evaluating kinase inhibitors. Built on HitHunter® assay technology, this kit is specifically optimized for Akt1/PKBalpha. It comes with all essential components, including recombinant Akt1/PKBalpha (Eurofins #14-276), a substrate, ATP, detection reagents, and assay plates. Each kit is accompanied by a Certificate of Analysis (COA), which outlines the recommended reaction conditions for accurate inhibitor detection. The supplied materials are sufficient to perform experiments covering two, six or twenty five 384-well assay plates.

Akt1, also known as protein kinase B alpha (PKBα), is a serine/threonine kinase that plays a central role in cell growth, proliferation, survival, and metabolism. Dysregulation of Akt1 signaling has been linked to multiple diseases, including cancer, diabetes, and cardiovascular disorders, making it a critical target in drug discovery and kinase inhibitor research. The availability of a ready-to-use Akt1 enzyme activity assay kit allows researchers to study this pathway with precision, accelerating the development of novel therapeutics targeting the PI3K/Akt signaling pathway.

Compound name
Kinase
Catalog number
44-276
Molecular formula
CAS
MW
Ka
Percent composition

Product specifications

Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
Solubility structure

Kinase Activity Assay Biological information

Background

The Kinase Enzyme Activity Assay Kit provides a reliable biochemical method for evaluating kinase inhibitors. Built on HitHunter® assay technology, this kit is specifically optimized for Akt1/PKBalpha. It comes with all essential components, including recombinant Akt1/PKBalpha (Eurofins #14-276), a substrate, ATP, detection reagents, and assay plates. Each kit is accompanied by a Certificate of Analysis (COA), which outlines the recommended reaction conditions for accurate inhibitor detection. The supplied materials are sufficient to perform experiments covering two, six or twenty five 384-well assay plates.

Akt1, also known as protein kinase B alpha (PKBα), is a serine/threonine kinase that plays a central role in cell growth, proliferation, survival, and metabolism. Dysregulation of Akt1 signaling has been linked to multiple diseases, including cancer, diabetes, and cardiovascular disorders, making it a critical target in drug discovery and kinase inhibitor research. The availability of a ready-to-use Akt1 enzyme activity assay kit allows researchers to study this pathway with precision, accelerating the development of novel therapeutics targeting the PI3K/Akt signaling pathway.

Target class
Kinase
Family
AGC
Subfamily
Serine/threonine kinase
Protein Name
Akt1
Protein Alias
PKBalpha; RAC-PK-alpha; c-Akt
Accession Number
GenBank M63167.1
UniProt Number
P31749
Gene Name
AKT1
Gene ID
207
Gene Aliases

AKT; PKB; RAC; PRKBA; PKB-ALPHA; RAC-ALPHA

Target Species
Human

Kinase Activity Assay Usage

Product Type
Enzyme Activity Assay Kits
Application
Drug Discovery & Development
Storage Conditions
1 year at -70°C
Usage disclaimer

For Research Use Only. These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.

Kinase Activity Assay Information

Assay Type
Enzymatic
Assay Measures
ADP Accumulation

Biological Information

Background

The Kinase Enzyme Activity Assay Kit provides a reliable biochemical method for evaluating kinase inhibitors. Built on HitHunter® assay technology, this kit is specifically optimized for Akt1/PKBalpha. It comes with all essential components, including recombinant Akt1/PKBalpha (Eurofins #14-276), a substrate, ATP, detection reagents, and assay plates. Each kit is accompanied by a Certificate of Analysis (COA), which outlines the recommended reaction conditions for accurate inhibitor detection. The supplied materials are sufficient to perform experiments covering two, six or twenty five 384-well assay plates.

Akt1, also known as protein kinase B alpha (PKBα), is a serine/threonine kinase that plays a central role in cell growth, proliferation, survival, and metabolism. Dysregulation of Akt1 signaling has been linked to multiple diseases, including cancer, diabetes, and cardiovascular disorders, making it a critical target in drug discovery and kinase inhibitor research. The availability of a ready-to-use Akt1 enzyme activity assay kit allows researchers to study this pathway with precision, accelerating the development of novel therapeutics targeting the PI3K/Akt signaling pathway.

Target class
Kinase
Family
AGC
Accession Number
GenBank M63167.1
Target Name
Akt1
Target Alias
PKBalpha; RAC-PK-alpha; c-Akt
Origin
Human
Theoretical MW
Affinity Tag

Product Specifications

Expression system
Purity
Purification method
Sample Buffer
Enzymatic
Specified activity
Application
Drug Discovery & Development
Storage conditions
1 year at -70°C
Usage disclaimer

For Research Use Only. These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.

Biological Information

Background

The Kinase Enzyme Activity Assay Kit provides a reliable biochemical method for evaluating kinase inhibitors. Built on HitHunter® assay technology, this kit is specifically optimized for Akt1/PKBalpha. It comes with all essential components, including recombinant Akt1/PKBalpha (Eurofins #14-276), a substrate, ATP, detection reagents, and assay plates. Each kit is accompanied by a Certificate of Analysis (COA), which outlines the recommended reaction conditions for accurate inhibitor detection. The supplied materials are sufficient to perform experiments covering two, six or twenty five 384-well assay plates.

Akt1, also known as protein kinase B alpha (PKBα), is a serine/threonine kinase that plays a central role in cell growth, proliferation, survival, and metabolism. Dysregulation of Akt1 signaling has been linked to multiple diseases, including cancer, diabetes, and cardiovascular disorders, making it a critical target in drug discovery and kinase inhibitor research. The availability of a ready-to-use Akt1 enzyme activity assay kit allows researchers to study this pathway with precision, accelerating the development of novel therapeutics targeting the PI3K/Akt signaling pathway.

Target class
Kinase
Family
AGC
Accession Number
GenBank M63167.1
Target Name
Akt1
Target Alias
PKBalpha; RAC-PK-alpha; c-Akt
Origin
Human
Theoretical MW
Affinity Tag

Product Specifications

Expression system
Purity
Purification method
Sample Buffer
Enzymatic
Specified activity
Application
Drug Discovery & Development
Storage conditions
1 year at -70°C
Usage disclaimer

For Research Use Only. These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.

Datasheets
44-276x Datasheet
User Manual
File N°2
Kinase Enzyme Activity Assay User Manual Rev 1
€ 950,00 EUR
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