b-catenin, activated
N-terminal c-Myc, 6His-tagged, recombinant human β-catenin full length, expressed by baculovirus in Sf21 insect cells.
No items found.
Biological information
Background
β-catenin is a multifunctional protein; it is a central component of the cadherin cell adhesion complex, regulating the control of cell adhesion and plays an essential role in the Wingless/Wnt signaling pathway that governs cell fate in early embryogenesis. The regulation of β-catenin turnover in the cells is governed by its phosphorylation status which allows the targeting of ubiquitin-dependent proteolysis. Altered levels of protein expression as well as genetic mutations of β-catenin have been implicated in a number of human cancers. Expected Purity 86% by SDS-PAGE and Coomassie blue staining. Purified using immobilized metal affinity chromatography.
Target class
Ubiquitin
Family
Substrate
Accession number
GenBank NM_001904
Target Name
b-catenin, activated
Target Alias
B-catenin, beta-catenin, ctnnb-a, ctnnb1-b, ctnnb1
Origin
Human
Theori. MW
91 kDa
Affinity tag
N-terminal c-Myc, 6His-tag
Product specifications
Expression system
expressed in insect cells
Purity
86%
Purification method
Immobilised metal affinity chromatography
Sample Buffer
0.49 mg/ml of enzyme in 25 mM MOPS pH 7.5, 5 mM MgCl2, 0.01% Tween 20, 1 mM ATP. Frozen solution.
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
1 year at -70°C
Usage disclaimer
For Research Only
Chemical data
Background
β-catenin is a multifunctional protein; it is a central component of the cadherin cell adhesion complex, regulating the control of cell adhesion and plays an essential role in the Wingless/Wnt signaling pathway that governs cell fate in early embryogenesis. The regulation of β-catenin turnover in the cells is governed by its phosphorylation status which allows the targeting of ubiquitin-dependent proteolysis. Altered levels of protein expression as well as genetic mutations of β-catenin have been implicated in a number of human cancers. Expected Purity 86% by SDS-PAGE and Coomassie blue staining. Purified using immobilized metal affinity chromatography.
Compound name
Ubiquitin
Catalog number
23-027
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Lipid Kinase Activity Assay Biological information
Background
β-catenin is a multifunctional protein; it is a central component of the cadherin cell adhesion complex, regulating the control of cell adhesion and plays an essential role in the Wingless/Wnt signaling pathway that governs cell fate in early embryogenesis. The regulation of β-catenin turnover in the cells is governed by its phosphorylation status which allows the targeting of ubiquitin-dependent proteolysis. Altered levels of protein expression as well as genetic mutations of β-catenin have been implicated in a number of human cancers. Expected Purity 86% by SDS-PAGE and Coomassie blue staining. Purified using immobilized metal affinity chromatography.
Target class
Ubiquitin
Family
Substrate
Subfamily
Protein Name
b-catenin
Protein Alias
B-catenin, beta-catenin, ctnnb-a, ctnnb1-b, ctnnb1
Accession Number
GenBank NM_001904
UniProt Number
Gene Name
Gene ID
Gene Aliases
Target Species
Human
Lipid Kinase Activity Assay Usage
Product Type
Application
Storage Conditions
1 year at -70°C
Usage disclaimer
Lipid Kinase Activity Assay Information
Assay Type
Assay Measures