CALIXAR™ C2B Kit
CALIXAR™ C2B Crystallization Kit
Biological information
This exciting new kit provides two varieties of stabilizing surfactants: Calixarenes 1-5 and fluorinated poly(tris) 6-8 and bis-glucose 9-11 surfactants. This new kit has been developed by CALIXAR SAS in partnership with the joint laboratory Chem2staB (C2B) at Avignon University, France. CALIXAR C2B derivatives have been designed & synthetized for their capacity to generate a network of salt bridges around the protein, in close proximity to the membrane domain with positively-charged residues located at the membrane-cytosol interface of the protein. They can also form a ‘cage’ around surface lysines, creating a ‘charge shield’. Fluorinated surfactants act in a very different manner to their hydrogenated equivalents due to the increased size of fluorine relative to hydrogen and its highly hydrophobic nature. Fluorinated surfactants are unable to extract proteins from the membrane, but can be useful in subsequent purification steps as they do not strip natural lipids and other co-factors from the proteins. In addition, the bulky fluorinated tailscannot penetrate into the interior and disrupt structure. Fluorinated surfactants often decrease non-specific aggregation and are thought to result in improved distribution on cryo-EM grids and better vitrification for cryo-EM data collection. They are also reported to increase crystallizability.
Product specifications
Chemical data
This exciting new kit provides two varieties of stabilizing surfactants: Calixarenes 1-5 and fluorinated poly(tris) 6-8 and bis-glucose 9-11 surfactants. This new kit has been developed by CALIXAR SAS in partnership with the joint laboratory Chem2staB (C2B) at Avignon University, France. CALIXAR C2B derivatives have been designed & synthetized for their capacity to generate a network of salt bridges around the protein, in close proximity to the membrane domain with positively-charged residues located at the membrane-cytosol interface of the protein. They can also form a ‘cage’ around surface lysines, creating a ‘charge shield’. Fluorinated surfactants act in a very different manner to their hydrogenated equivalents due to the increased size of fluorine relative to hydrogen and its highly hydrophobic nature. Fluorinated surfactants are unable to extract proteins from the membrane, but can be useful in subsequent purification steps as they do not strip natural lipids and other co-factors from the proteins. In addition, the bulky fluorinated tailscannot penetrate into the interior and disrupt structure. Fluorinated surfactants often decrease non-specific aggregation and are thought to result in improved distribution on cryo-EM grids and better vitrification for cryo-EM data collection. They are also reported to increase crystallizability.