Our core business is the isolation of native, functional and integral membrane proteins, in addition to deorphanization and identification of membrane protein partners.
CALIXAR offers an unique and patented technological platform to isolate in solution, native full-length membrane proteins, while keeping their structural and functional integrity. This approach allows to guide you in your projects from expression, extraction and purification.
The combination of our expertise in biochemistry of integral membrane proteins (IMPs) and the chemistry of stabilizing reagents used for isolating IMPs, put us in an unique position to facilitate your drug discovery projects.
“ Designing the best chemistry to make the best biochemistry”
Conventional approaches tend to modify the protein through deletions or point mutations to make it adapted to the environment. Instead, CALIXAR’s approach modifies the chemical environment to match the chemistry of the proteins/antigens.
Therefore, our process allows the conservation of the functional and structural integrities of the targets/ antigens. This has a significant positive impact on the quality of the protein and on the success of drug discovery applications.
We can start either from your gene sequence, primary cells, recombinant cells, tissues or organs,…
Our technology is applicable to every kind of membrane proteins (GPCRs, Ion Channels, Transporters, Other Antigens, among others) and also to soluble proteins which are difficult to stabilize in solution.
Our unique chemical facilities enable us to continuously design new reagents (detergents/ surfactants,…), to identify, isolate, stabilize, and/ or crystallize your targets in the best conditions.
We offer a variety of services: deorphanization, identification, expression, extraction, purification, stabilization, functional assays, X-ray crystallography and detergent quantification.
Highly compatible expertise with downstream processing (DSP) and upstream processing (USP – expression part).